THE ANTIGENIC STRUCTURE OF THE INFLUENZA-VIRUS A/PR/8/34 HEMAGGLUTININ (H-1 SUBTYPE)
THE ANTIGENIC STRUCTURE OF THE INFLUENZA-VIRUS A/PR/8/34 HEMAGGLUTININ (H-1 SUBTYPE)
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DOI：
10.1016/0092-8674(82)90135-0
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
GERHARD, W
中科院分区：
文献类型：
--
作者：
CATON, AJ;BROWNLEE, GG;GERHARD, W
构建了甲型流感病毒A/PR/8/34血凝素的操作抗原图谱，该图谱显示存在5个免疫显性抗原区域，这些区域表现出不同程度的操作连锁。通过鉴定在每个位点发生抗原改变的突变病毒中改变的氨基酸残基，确定了这些位点。将抗原特征与H3亚型血凝素的三维结构进行比较，结果表明抗原位点对应于蛋白质表面4个在拓扑结构上不同的区域。其中一个位点是在血凝素单体中相距较远的两个区域在组装的三聚体中结合时形成的。这些位点相对于H3亚型血凝素所提出位点的位置表明，碳水化合物调节血凝素特定区域的抗原性。
An operational antigenic map of the hemagglutinin of influenza virus A/PR/8/34, which indicates the presence of 5 immunodominant antigenic regions exhibiting various degrees of operational linkage, was constructed. These sites have been located by the identification of changed amino acid residues in mutant viruses that are antigenically altered at each site. Comparison of the antigenic features with the 3-dimensional structure of the H3 subtype hemagglutinin shows that the antigenic sites correspond to 4 topographically distinct regions of the surface of the protein. One of the sites is formed when 2 regions that are widely separated in the hemagglutinin monomer associate in the assembled trimer. The location of the sites relative to those proposed for the H3 subtype hemagglutinin suggests that carbohydrate modulates the antigenicity of specific regions of the hemagglutinin.