Interaction of S-SCAM with neural plakophilin-related armadillo-repeat protein/δ-catenin
Interaction of S-SCAM with neural plakophilin-related armadillo-repeat protein/δ-catenin
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DOI：
10.1006/bbrc.1999.0364
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发表时间：
1999-03-24
影响因子：
3.1
通讯作者：
Takai, Y
中科院分区：
文献类型：
--
作者：
Ide, N;Hata, Y;Takai, Y
突触支架分子(Synaptic Scaffolding分子，S-SCAM)是一种含有多个PDZ结构域的蛋白，它与细胞黏附分子神经连接素和N-甲基-天冬氨酸受体相互作用。在本研究中，我们搜索了S-SCAM相互作用蛋白，获得了一个与S-SCAM相互作用的蛋白，在三种不同的无细胞分析系统中通过其羧基末端与S-SCAM的最后一个PDZ结构域结合，并与S-SCAM免疫共沉淀，定位于大鼠海马神经元的兴奋性突触。NPRAP/Delta-catenin可能通过与S-SCAM的相互作用而参与突触连接的分子组织，(C)1999学术出版社。
Synaptic scaffolding molecule (S-SCAM) is a multiple PDZ domain-containing protein, which interacts with neuroligin, a cell adhesion molecule, and the NMDA receptor. In this study, we searched for S-SCAM-interacting proteins and obtained a neural plakophilin-related armadillo-repeat protein (NPRAP)/delta-catenin, NPRAP/delta-catenin bound to the last PDZ domain of S-SCAM via its carboxyl-terminus in three different cell-free assay systems, was coimmunoprecipitated with S-SCAM from rat crude synaptosomes, and was localized at the excitatory synapses in rat hippocampal neurons. NPRAP/delta-catenin may be implicated in the molecular organization of synaptic junctions through the interaction with S-SCAM, (C) 1999 Academic Press.