Evidence for a genetic component in Sudden Infant Death Syndrome
Evidence for a genetic component in Sudden Infant Death Syndrome
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DOI：
10.1046/j.1365-2214.2002.00008.x
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发表时间：
2002-09-01
影响因子：
1.9
通讯作者：
Blackwell, CC
中科院分区：
文献类型：
--
作者：
Gordon, AE;MacKenzie, DAC;Blackwell, CC
越来越多的证据表明，一些婴儿猝死综合症（SIDS）婴儿会引发炎症反应，并且这些反应受到基因控制。本研究的目的是调查 SIDS 父母 (n = 41) 的细胞因子反应与对照供体 (n = 61) 显着不同的假设。使用来自大肠杆菌的葡萄球菌毒素 TSST-1 和 LPS 刺激血样，并评估 TNF、IL-1、IL-6、IFN 和 IL-10 的产生。 SIDS 父母对 TSST-1 (P < 0.02) 和 LPS (P < 0.002) 的反应比对照组产生更高水平的 IL-1。与 LPS 相比，SIDS 父母对 TSST-1 产生的 IFN 水平更高（P < 0.001），尽管对 LPS 的反应中，SIDS 父母的 IFN（P = 0.0008）和 IL-6（P < 0.0002）反应低于对照组。对于 TNF 和 IL-10，除非考虑吸烟的影响，否则两组之间几乎没有差异。作为这项工作的一部分，使用来自 SIDS 父母 (n = 10)、对照捐赠者 (n = 10) 和孟加拉国受试者 (n = 10) 的 DNA 进行了一项小型试点基因分型研究。分别在 40%、15.4% 和 0% 的供体中发现了 IFN 多态性 (3/3)。已在 SIDS 婴儿中发现葡萄球菌毒素，因此本研究强调了评估 IL-1 水平的重要性。确定细胞因子多态性并考虑这些因素与高、中、低风险种族群体中吸烟等环境因素之间的相互作用将有助于确定这些因素对婴儿 SIDS 易感性的影响。
There is increasing evidence that inflammatory responses have been elicited in some Sudden Infant Death Syndrome (SIDS) infants and that these responses are under genetic control. The objective of this study was to investigate the hypothesis that the cytokine responses of SIDS parents (n = 41) differed significantly from control donors (n = 61). Blood samples were stimulated with the staphylococcal toxin TSST-1 and LPS from Eschericia coli and assessed for production of TNF, IL-1, IL-6, IFN and IL-10. In response to TSST-1 (P < 0.02) and LPS (P < 0.002), SIDS parents produced higher levels of IL-1 than the controls. SIDS parents produced higher levels of IFN in response to TSST-1 compared to LPS (P < 0.001) although in response to LPS, the IFN (P = 0.0008) and IL-6 (P < 0.0002) responses of the SIDS parents were lower than those of the controls. For TNF and IL-10, there was little difference between the two groups unless the effect of smoking was considered. As part of this work, a small pilot genotyping study was carried out using DNA from SIDS parents (n = 10), control donors (n = 10) and Bangladeshi subjects (n = 10). An IFN polymorphism (3/3) was found in 40%, 15.4% and 0% of donors respectively. Staphylococcal toxins have been identified in SIDS infants therefore this study highlights the importance of assessing IL-1 levels. Determination of cytokine polymorphisms and consideration of interactions between these and environmental factors such as smoking in high, average and low risk ethnic groups will assist in establishing the contribution of these factors to an infant's susceptibility to SIDS.