Cytotoxicity, Adhesion and Invasion ofClostridium septicumin Cultured Human Epithelial Cells (CACO-2, HEp-2): Pathological Significance of Swarm Cell Differentiation: CLINICAL MEDICINE/PATHOGENESIS
Cytotoxicity, Adhesion and Invasion ofClostridium septicumin Cultured Human Epithelial Cells (CACO-2, HEp-2): Pathological Significance of Swarm Cell Differentiation: CLINICAL MEDICINE/PATHOGENESIS
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败血梭菌培养的人上皮细胞（CACO-2、HEp-2）的细胞毒性、粘附和侵袭：群细胞分化的病理意义：临床医学/发病机制
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
G. Macfarlane
中科院分区：
文献类型：
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作者：
L. M. Wilson;G. Macfarlane
摘要败血梭菌NCTC282对CACO-2和Hep-2细胞有细胞毒作用。然而，Hep-2细胞比CACO-2细胞对TOC的耐药性更强。败血症细胞毒素。短小可移动的杆状细菌比成群的细菌具有更强的细胞毒性。细菌黏附只发生在Hep-2上，短的活动杆状细菌黏附的数量明显多于成群的细菌。通过机械剪切去除细菌鞭毛对粘附性几乎没有影响，表明这些表面结构不起粘附素的作用。在HEP-2细胞单层中加入多种单糖(蔗糖、D-岩藻糖、L-岩藻糖、甘露糖)对细菌黏附几乎没有影响，但麦芽糖对细菌黏附有50%的抑制作用。C.侵袭Hep-2细胞。败血症发生在可移动的短杆上，而不是聚集在一起的细菌，这表明这种微生物感染的初始阶段可能受到不同细胞形式附着和入侵上皮细胞的能力的影响。由于其对细胞的毒性、黏附和上皮细胞的侵袭作用。败血症依赖于细菌的形态，机体对环境因素的反应分化为群体细胞会影响感染的发病机制。
Abstract Clostridium septicumNCTC 282 was cytotoxic for CACO-2 and HEp-2 cells. However, HEp-2 cells were more resistant than CACO-2 cells toC. septicumcytotoxin. Short motile rod forms were considerably more cytotoxic than swarming bacteria. Bacterial adhesion only occurred with HEp-2, and short motile rod forms adhered in significantly greater numbers than swarming bacteria. Removal of bacterial flagella by mechanical shearing had little effect on adherence, demonstrating that these surface structures did not act as adhesins. Addition of a variety of monosaccharides (sucrose, D -fucose, L -fucose, mannose) to HEp-2 cell monolayers had little effect on bacterial adhesion, although maltose effected a 50% reduction. Invasion of HEp-2 cells byC. septicumoccurred with short motile rods, but not with swarming bacteria, suggesting that the initial stages of infection by this organism may be affected by the abilities of different cell forms to adhere to and invade epithelial cells. Since cytotoxicity, adherence and epithelial cell invasion byC. septicumis dependent on bacterial form, differentiation of the organism into swarm cells in response to environmental factors will influence the pathogenesis of infection.