Ebola virus can be effectively neutralized by antibody produced in natural human infection
Ebola virus can be effectively neutralized by antibody produced in natural human infection
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DOI：
10.1128/jvi.73.7.6024-6030.1999
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发表时间：
1999-07-01
影响因子：
5.4
通讯作者：
Burton, DR
中科院分区：
文献类型：
--
作者：
Maruyama, T;Rodriguez, LL;Burton, DR
丝状病毒抗体的活性知之甚少，但对疫苗设计和被动预防具有重要意义。为了调查这一活动，一组针对埃博拉病毒抗原的重组人单克隆抗体是从噬菌体展示文库中分离的，所述噬菌体展示文库由来自1995年刚果基奎特埃博拉病毒爆发中感染恢复的供体的RNA构建。核蛋白（NP），包膜糖蛋白（GP），分泌包膜糖蛋白（sGP）的抗体反应的特点是通过免疫荧光和放射免疫沉淀试验。与sGP反应强而与GP反应弱的4株抗体和与NP反应弱的2株抗体不具有中和性。GP特异性抗体在0.4 μ g/ml时中和埃博拉病毒至50%作为重组Fab片段，在0.3 μ g/ml时中和至50%（在2.6 μ g/ml时90%）作为相应的完整免疫球蛋白G1分子。这些研究表明，埃博拉病毒感染时会产生中和抗体，尽管频率可能相对较低。中和抗体可用于疫苗设计和作为针对埃博拉病毒感染的预防剂。
The activity of antibodies against filoviruses is poorly understood but has important consequences for vaccine design and passive prophylaxis. To investigate this activity,. panel of recombinant human monoclonal antibodies to Ebola virus antigens was isolated from phage display libraries constructed from RNA from donors who recovered from infection in the 1995 Ebola virus outbreak in Kikwit, Democratic Republic of Congo. Antibodies reactive with nucleoprotein (NP), envelope glycoprotein (GP), and secreted envelope glycoprotein (sGP) were characterized by immunofluorescence and radioimmunoprecipitation assays. Four antibodies reacting strongly with sGP and weakly with GP and two antibodies reacting with NP were not neutralizing. An antibody specific for GP neutralized Ebola virus to 50% at 0.4 mu g/ml as the recombinant Fab fragment and to 50% at 0.3 mu g/ml (90% at 2.6 mu g/ml) as the corresponding whole immunoglobulin G1 molecule. The studies indicate that neutralizing antibodies are produced in infection by Ebola virus although probably at a relatively low frequency. The neutralizing antibody may be useful in vaccine design and as a prophylactic agent against Ebola virus infection.