Anaerobic reductive dechlorination of 1-chloro-1-fluoroethene to track the transformation of vinyl chloride.
Anaerobic reductive dechlorination of 1-chloro-1-fluoroethene to track the transformation of vinyl chloride.
复制标题
1-氯-1-氟乙烯的厌氧还原脱氯追踪氯乙烯的转化。
DOI：
10.1021/es0498383
复制
发表时间：
2004
影响因子：
11.4
通讯作者：
Semprini,Lewis
中科院分区：
文献类型：
--
作者：
Pon,George;Semprini,Lewis
以1-氯-1-氟乙烯（1，1-CFE）为示踪剂，研究了氯乙烯（VC）厌氧转化的反应性。使用从Corvallis，OR的Evanite工厂获得的富集培养物进行1，1-CFE和VC转化的分批动力学研究。该菌能将三氯乙烯（TCE）经顺式二氯乙烯（c-DCE）和VC完全转化为乙烯。该培养物还将氟化类似物，如三氯氟乙烯（TCFE）转化为氟乙烯（FE）作为最终产物。氟化类似物的转化序列与具有相同程度的氯取代的氯化乙烯所实现的转化序列相关。例如，由TCFE转化形成的主要CFE异构体1，1-CFE的产生与由TCE转化产生的VC相关。由于1，1-CFE及其产物FE具有独特的分析特征，因此1，1-CFE可用作反应性原位示踪剂来评估VC转化潜力。VC和1，1-CFE的半饱和常数（Ks）分别为63和87 μM，而最大利用率（kmaxX）分别为334和350 μM/d。乙炔抑制VC和1，1-CFE的转化。一个竞争性抑制模型与独立measuredKsvalues作为抑制常数预测VC和1，1-CFE的转化率时，这两种化合物都存在。还使用三种不同的培养物测试了1，1-CFE转化。在所有培养物中，1，1-CFE转化与VC转化为乙烯相关，转化率相当。结果表明，1，1-CFE是一个很好的反应性替代品，用于评估VC转化率。
1-Chloro-1-fluoroethene (1,1-CFE) was studied as a reactive tracer to quantify the anaerobic transformation of vinyl chloride (VC). Batch kinetic studies of 1,1-CFE and VC transformation were performed with an enrichment culture obtained from the Evanite site in Corvallis, OR. The culture is capable of completely transforming trichloroethene (TCE) throughcis-dichloroethene (c-DCE) and VC to ethene. The culture also transforms fluorinated analogues, such as trichlorofluoroethene (TCFE), to fluoroethene (FE) as a final product. The transformation sequence of the fluorinated analogue was correlated with that achieved for the chlorinated ethene with the same degree of chloride substitution. For example, the production of 1,1-CFE, the major CFE isomer formed from TCFE transformation, was correlated with the production of VC from TCE transformation. Since the 1,1-CFE and its product, FE, have a distinct analytical signature, 1,1-CFE may be used as a reactive in situ tracer to evaluate the VC transformation potential. The half-saturated constants (Ks) of VC and 1,1-CFE were 63 and 87 μM, respectively, while similar maximum utilization rates (kmaxX) of 334 and 350 μM/d were achieved. Acetylene inhibited both VC and 1,1-CFE transformation. A competitive inhibition model with the independently measuredKsvalues used as the inhibition constants predicted rates of transformation of both VC and 1,1-CFE when both compounds were present. 1,1-CFE transformation was also tested with three different cultures. With all the cultures, 1,1-CFE transformation was associated with VC transformation to ethene, and the rates of transformation were comparable. The results demonstrated that 1,1-CFE was a good reactive surrogate for evaluating the rates of VC transformation.