Phase separation of lipid membranes analyzed with high-resolution secondary ion mass spectrometry
Phase separation of lipid membranes analyzed with high-resolution secondary ion mass spectrometry
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DOI：
10.1126/science.1130279
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发表时间：
2006-09-29
期刊：
影响因子：
56.9
通讯作者：
Boxer, Steven G.
中科院分区：
文献类型：
--
作者：
Kraft, Mary L.;Weber, Peter K.;Boxer, Steven G.
膜成分的横向变化被认为在许多细胞事件中发挥核心作用，但在数十至数百纳米的长度尺度上探测膜成分和组织一直很困难。我们提出了一种高分辨率成像二次离子质谱技术，以类似于 100 纳米的横向分辨率揭示相分离膜内的脂质分布。通过使用同位素标记来识别每个分子种类，获得了有关小脂质域内化学成分的定量信息。在某些领域内检测到成分变化。
Lateral variations in membrane composition are postulated to play a central role in many cellular events, but it has been difficult to probe membrane composition and organization on length scales of tens to hundreds of nanometers. We present a high-resolution imaging secondary ion mass spectrometry technique to reveal the lipid distribution within a phase-separated membrane with a lateral resolution of similar to 100 nanometers. Quantitative information about the chemical composition within small lipid domains was obtained with the use of isotopic labels to identify each molecular species. Composition variations were detected within some domains.