Determining the protein repertoire of Cryptosporidium parvum sporozoites
Determining the protein repertoire of Cryptosporidium parvum sporozoites
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DOI：
10.1002/pmic.200700804
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发表时间：
2008-04-01
期刊：
影响因子：
3.4
通讯作者：
Wastling, Jonathan M.
中科院分区：
文献类型：
--
作者：
Sanderson, Sanya J.;Xia, Dong;Wastling, Jonathan M.
细胞内寄生虫微小隐孢子虫的基因组最近已经被测序，但这种重要的人畜共患胃肠道病原体侵袭阶段的蛋白质表达数据有限。本文对微小隐孢子虫体外卵囊/子孢子制剂的表达蛋白质谱进行了综合分析。使用了三个独立的蛋白质组平台，产生了超过4800个单独的蛋白质鉴定，代表了1237个非冗余蛋白质，相当于预测蛋白质组的30%。将多肽数据映射到微小隐孢子虫基因组上的相应位置，并开发了一个可公开访问的蛋白质组数据接口，用于在CryptoDB(http://cryptodb.org).)上进行数据挖掘和可视化这些数据为改进基因组注释、验证预测的假设蛋白质和识别当前基因模型未预测的蛋白质提供了及时和有价值的资源。这些数据表明，可能对寄生虫的入侵和细胞内建立起重要作用的蛋白质的表达，包括表面蛋白、残留线粒体的成分和顶端细胞器。将表达的蛋白质组与现有的转录数据进行比较，发现只有微弱的相关性。对于大约一半的蛋白质组来说，功能和结构信息有限，这突显了目前对隐孢子虫生物学理解的局限性。
The genome of the intracellular parasite Cryptosporidium parvum has recently been sequenced, but protein expression data for the invasive stages of this important zoonotic gastrointestinal pathogen are limited. In this paper a comprehensive analysis of the expressed protein repertoire of an excysted oocyst/sporozoite preparation of C. parvum is presented. Three independent proteome platforms were employed which yielded more than 4800 individual protein identifications representing 1237 nonredundant proteins, corresponding to similar to 30% of the predicted proteome. Peptide data were mapped to the corresponding locations on the C. parvum genome and a publicly accessible interface for proteome data was developed for data-mining and visualisation at CryptoDB (http://cryptodb.org). These data provide a timely and valuable resource for improved annotation of the genome, verification of predicted hypothetical proteins and identification of proteins not predicted by current gene models. The data indicated the expression of proteins likely to be important to the invasion and intracellular establishment of the parasite, including surface proteins, constituents of the remnant mitochondrion and apical organelles. Comparison of the expressed proteome with existing transcriptional data indicated only a weak correlation. For approximately half the proteome there was limited functional and structural information, highlighting the limitations in the current understanding of Cryptosporidium biology.