Identification of novel adipokines differential regulated in C57BL/Ks and C57BL/6
Identification of novel adipokines differential regulated in C57BL/Ks and C57BL/6
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DOI：
10.3109/13813455.2014.970197
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发表时间：
2014-12-01
影响因子：
3
通讯作者：
Lehr, Stefan
中科院分区：
文献类型：
--
作者：
Hartwig, Sonja;Goeddeke, Simon;Lehr, Stefan
内脏性肥胖与代谢紊乱有关，但其病理生理机制尚不清楚。一个可能的联系可能是释放各种信号和介质蛋白，称为脂肪因子。我们的假设是，依赖于遗传背景因素的释放可能会引发原发性疾病的易感性。本研究从两个代谢健康的小鼠品系，即C57 BL/Ks（BKS）和C57 BL/6（C57）的内脏脂肪组织中释放脂肪因子，其中前者的遗传背景在代谢挑战后对发展糖尿病更敏感。使用液相色谱（LC）-电喷雾电离（ESI）-MS/MS，产生包含597种脂肪因子的参考图谱（http：www.diabesityprot.org）。35个脂肪因子，包括6个以前没有描述的，差异释放的小鼠品系之间。最值得注意的是BKS小鼠中脂联素结合蛋白T-钙粘蛋白（CAD 13）的释放减少。这一观察结果强调了分泌组分析在揭示遗传多样性和生活方式之间复杂相互作用方面的重要性。
Visceral adiposity is associated with metabolic disorders, but little is known on the underlying pathophysiological mechanism. One possible link might be the release of various signalling and mediator proteins, named adipokines. Our hypothesis was that dependent on genetic background factors are released which might trigger a primary disease susceptibility. This study characterizes the adipokines released from visceral adipose tissue from two metabolic healthy mouse strains, i.e. C57BL/Ks (BKS) and C57BL/6 (C57), of which the former genetic background is more sensitive to develop diabetes following metabolic challenge. Using liquid chromatography (LC)-electrospray ionization (ESI)-MS/MS, a reference map comprising 597 adipokines was generated (http://www.diabesityprot.org). Thirty-five adipokines, including six not previously described ones, were differentially released between the mouse strains. Most notable is the reduced release of the adiponectin-binding protein T-Cadherin (CAD13) in BKS mice. This observation highlights the importance of secretome profiling in unravelling the complex interplay between genetic diversity and lifestyle.