The use of Listeria monocytogenes as a DNA delivery vector for cancer gene therapy.
The use of Listeria monocytogenes as a DNA delivery vector for cancer gene therapy.
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DOI：
10.4161/bbug.1.4.11725
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发表时间：
2010-07-01
期刊：
影响因子：
--
通讯作者：
Gahan, Cormac G M
中科院分区：
文献类型：
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作者：
Tangney, Mark;van Pijkeren, Jan Peter;Gahan, Cormac G M
单核细胞增多性李斯特菌是一种胞内病原体，能裂解感染细胞的吞噬空泡，在宿主细胞质中增殖，并能主动进入邻近细胞。因此，这种病原体非常适合作为将DNA运送到靶细胞的载体来开发，因为其生命周期有利于通过载体扩增的细胞靶向和细胞间传播的可能性。我们最近在小鼠肿瘤生长模型中证明了单核细胞增多性乳杆菌的DNA转移。我们的方法利用了一种氨苄西林敏感的单核细胞增生性李斯特菌菌株，这种菌株可以通过全身给药来裂解，以促进受感染的哺乳动物细胞释放质粒DNA进行表达。在这里，我们讨论这项技术的含义和未来改进该系统的潜力。
Listeria monocytogenes is an intracellular pathogen that lyses the phagosomal vacuole of infected cells, proliferates in the host cell cytoplasm and can actively enter adjacent cells. The pathogen is therefore well suited to exploitation as a vector for the delivery of DNA to target cells as the lifecycle favors cellular targeting with vector amplification and the potential for cell-to-cell spread. We have recently demonstrated DNA transfer by L. monocytogenes in growing tumors in murine models. Our approach exploited an ampicillin sensitive stain of L. monocytogenes which can be lysed through systemic administration of ampicillin to facilitate release of plasmid DNA for expression by infected mammalian cells. Here, we discuss the implications of this technology and the potential for future improvements of the system.