Isolation and identification of a cold-inducible gene encoding a putative DRE-binding transcription factor from Festuca arundinacea.
Isolation and identification of a cold-inducible gene encoding a putative DRE-binding transcription factor from Festuca arundinacea.
复制标题
DOI：
10.1016/j.plaphy.2005.01.015
复制
发表时间：
2005-03
期刊：
影响因子：
--
通讯作者：
Mingjuan Tang;Shiyou Lü;Y. Jing;Xiangjuan Zhou;Jingwen Sun;S. Shen
中科院分区：
文献类型：
--
作者：
Mingjuan Tang;Shiyou Lü;Y. Jing;Xiangjuan Zhou;Jingwen Sun;S. Shen
利用RACE-PCR技术从高羊茅（Festuca arundinacea Schreb）幼苗中克隆到一个新的DREB结合蛋白基因FaDREB 1，该基因编码一个AP 2/ERFBP类转录因子。其cDNA序列为988 bp，编码216个氨基酸，预测分子量为23.479 kDa，pI为4.70。对蛋白Blast数据的搜索显示，该蛋白可以被分类为DNA结合蛋白的AP 2/EREBP家族的典型成员。FaDREB 1基因的组织器官特异性表达模式表明，其转录本在叶片和叶鞘中表达丰富，而在根中表达较少。Southern杂交分析表明该基因为多拷贝基因。其mRNA积累曲线表明，其表达受冷处理的强烈诱导，干旱和盐胁迫的弱诱导，但不响应阿坝处理。推测FaDREB 1蛋白可能通过ABA非依赖性途径参与植物对冷胁迫的应答过程。
A new DRE-binding protein gene FaDREB1 encoded for an AP2/ERFBP-type transcription factor was isolated by RACE-PCR from Festuca arundinacea Schreb seedlings. Its cDNA was sequenced with 988 bp, from which a protein with 216 amino acid residues was deduced with a predicted molecular mass of 23.479 kDa and a pI of 4.70. A search of the Protein Blast data revealed that this protein can be classified as a typical member of the AP2/EREBP family of DNA-binding proteins. The tissue organ-specific expression pattern of the FaDREB1 gene showed that its transcripts were abundant in leaves and leaf sheaths, and scarce in roots. Southern blot analysis indicated that it is a multiple-copy gene. Its mRNA accumulation profiles made clear that its expression was strongly induced by cold treatment, weakly induced by drought and salt stress, but did not respond to ABA treatment. It was concluded that the protein FaDREB1 may be involved in the process of plant response to cold stress through an ABA-independent pathway.