Chitosan-coated triangular silver nanoparticles as a novel class of biocompatible, highly effective photothermal transducers for in vitro cancer cell therapy
Chitosan-coated triangular silver nanoparticles as a novel class of biocompatible, highly effective photothermal transducers for in vitro cancer cell therapy
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DOI：
10.1016/j.canlet.2011.06.022
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发表时间：
2011-12-08
期刊：
影响因子：
9.7
通讯作者：
Astilean, Simion
中科院分区：
文献类型：
--
作者：
Boca, Sanda C.;Potara, Monica;Astilean, Simion
纳米技术当今的相关方向之一与纳米医学有关，特别是与光和纳米颗粒在癌症早期诊断和有效治疗中的使用有关。贵金属纳米粒子可以在激光照射下作为有效的光热传感器来触发肿瘤的局部高热。在这项工作中，我们报告了新合成的壳聚糖包被的银纳米三角形（Chit-AgNT）在近红外（NIR）中具有强共振，作为光热剂对抗一系列人类非小肺癌细胞（NCI-H460）的性能。热疗实验是通过 Ti:Sapphire 激光器在 800 nm 波长下激发纳米粒子负载的细胞来进行的。我们发现，Chit-AgNTs 存在下的细胞死亡率高于硫醇化聚乙二醇封端金纳米棒 (PEG-AuNRs)（一种用作参考的常见热疗剂）存在下的细胞死亡率，而在相同的照射条件下，对对照样品（不含纳米粒子的细胞）没有观察到破坏性影响。此外，我们进行了细胞毒性测定，发现 Chit-AgNT 可以被细胞有效摄取，同时对健康人胚胎细胞 (HEK) 表现出良好的生物相容性，这对于任何体内应用都是至关重要的。我们的研究结果揭示了一类新型的生物相容性等离子体纳米颗粒，具有作为抗癌有效光疗剂的巨大潜力。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
One of the relevant directions that nanotechnology is taking nowadays is connected with nanomedicine and specifically related to the use of light and nanoparticles in early diagnosis and effective therapeutics of cancer. Noble-metal nanoparticles can act under laser irradiation as effective photothermal transducers for triggering localized hyperthermia of tumors. In this work we report the performance of newly synthesized chitosan-coated silver nanotriangles (Chit-AgNTs) with strong resonances in near-infrared (NIR) to operate as photothermal agents against a line of human non-small lung cancer cells (NCI-H460). The hyperthermia experiments were conducted by excitation of nanoparticles-loaded cells at 800 nm wavelength from a Ti:Sapphire laser. We found that the rate of cell mortality in the presence of Chit-AgNTs is higher than in the presence of thiolated poly(ethylene) glycol capped gold nanorods (PEG-AuNRs) - a common hyperthermia agent used as reference-, while no destructive effects were noticed on the control sample (cells without nanopartides) under identical irradiation conditions. Additionally, we conducted cytotoxicity assays and found Chit-AgNTs to be efficiently uptaken by the cells while exhibiting good biocompatibility for healthy human embryonic cells (HEK), which is essential for any in vivo applications. Our results reveal a novel class of biocompatible plasmonic nanoparticles with high potential to be implemented as effective phototherapeutic agents in the battle against cancer. (C) 2011 Elsevier Ireland Ltd. All rights reserved.