Chorionic gonadotropin sensitive adenylate cyclase in human term placenta.
Chorionic gonadotropin sensitive adenylate cyclase in human term placenta.
复制标题
人足月胎盘中绒毛膜促性腺激素敏感的腺苷酸环化酶。
DOI：
10.1210/jcem-36-6-1104
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
R. Jaffe
中科院分区：
文献类型：
--
作者：
K. Menon;R. Jaffe
在17 mM Mg2+和7.0 mM F−离子存在下，人足月胎盘均浆和洗涤颗粒中的腺苷酸环化酶活性最大。对Mg2+的要求可以用Mn2+代替，但不能用其他金属离子代替。腺苷酸环化酶活性在体外匀浆制备中不受hCG的刺激。然而，使用切碎的胎盘制剂，hCG刺激14c -腺嘌呤并入14c -环AMP。hCG对胎盘环AMP形成的刺激作用是浓度依赖性和特异性的。这些实验表明hCG可能在胎盘功能中具有循环amp介导的作用。
Adenylate cyclase activity in a homogenate and washed particles of human term placenta proceeded maximally in the presence of 17 mM Mg2+ and 7.0 mM F− ion. The requirements for Mg2+ can be substituted by Mn2+, but not by other metal ions. Adenylate cyclase activity was not stimulated in vitro in a homogenate preparation by the addition of hCG. However, using a minced placental preparation, hCG stimulated the incorporation of 14C-adenine into 14C-cyclic AMP. The stimulatory effect of hCG on placental cyclic AMP formation was concentration dependent and specific. These experiments suggest that hCG may have a cyclic AMP-mediated role in placental function.