FIBULIN, A NOVEL PROTEIN THAT INTERACTS WITH THE FIBRONECTIN RECEPTOR-BETA SUBUNIT CYTOPLASMIC DOMAIN
FIBULIN, A NOVEL PROTEIN THAT INTERACTS WITH THE FIBRONECTIN RECEPTOR-BETA SUBUNIT CYTOPLASMIC DOMAIN
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DOI：
10.1016/0092-8674(89)90097-4
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发表时间：
1989-08-25
期刊：
影响因子：
64.5
通讯作者：
RUOSLAHTI, E
中科院分区：
文献类型：
--
作者：
ARGRAVES, WS;DICKERSON, K;RUOSLAHTI, E
通过在代表纤连蛋白受体β胞质结构域的合成肽上进行亲和层析，从组织和细胞提取物中分离100 kd蛋白质。亚单位100 kd的蛋白质也结合天然纤连蛋白受体，这种结合可以用EDTA逆转。钙可能是结合所需的二价阳离子，因为发现100 kd蛋白质结合45 Ca 2+。100 kd蛋白的N-末端氨基酸序列与蛋白质数据库中的任何序列都不相似。在纤连蛋白上培养的细胞的免疫荧光染色显示100 Kd蛋白与纤连蛋白受体β一致。亚基在基板接触的网站。因此，这种蛋白质，我们称之为纤蛋白，在体外与纤连蛋白受体相互作用，并在体内与受体结合。纤维蛋白是粘附受体和细胞骨架之间相互作用的潜在介质。
A 100 kd protein was isolated from tissue and cell extracts by affinity chromatography on a synthetic peptide representing the cytoplasmic domain of the fibronectin receptor .beta. subunit. The 100 kd protein also bound to native fibronectin receptor, and this binding could be reversed with EDTA. Calcium may be the divalent cation required for the binding since the 100 kd protein was found to bind 45Ca2+. The N-terminal amino acid sequence of the 100 kd protein was not similar to any sequence in a protein data base. Immunofluorescent staining of cells cultured on fibronectin showed the 100 Kd protein coinciding with the fibronectin receptor .beta. subunit in sites of substrate contact. Therefore this protein, which we term fibulin, interacts with the fibronectin receptor in vitro and associates with the receptor in vivo. Fibulin is a potential mediator of interactions between adhesion receptors and the cytoskeleton.