Structure and in vitro transcription of a human H4 histone gene.
Structure and in vitro transcription of a human H4 histone gene.
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人类 H4 组蛋白基因的结构和体外转录。
DOI：
10.1093/nar/11.20.7069
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发表时间：
1983
影响因子：
14.9
通讯作者：
Stein,J
中科院分区：
文献类型：
--
作者：
Sierra,F;Stein,G;Stein,J
分离了人H4组蛋白基因，并确定了mRNA编码区以及5 '和3'侧翼区的核苷酸序列。在该基因中未发现插入序列。在H4组蛋白编码区的上游和下游鉴定了一系列在组蛋白基因和/或其他基因中被指定了推定的调控作用的序列。用BAL-31核酸酶消化H4组蛋白基因5′侧翼序列，构建缺失突变体，并在体外转录系统中进行检测。TATA盒上游区域不需要体外位点特异性启动。结果表明，位于编码区3′端下游的序列可能影响H4组蛋白基因的体外转录。
A human H4 histone gene was isolated and the nucleotide sequences of the mRNA coding as well as the 5′ and 3′ flanking regions were determined. No intervening sequences were found in this gene. A series of sequences which have been assigned putative regulatory roles in histone genes and/or in other genes were identified both upstream and downstream from the H4 histone protein coding region. Deletion mutants were constructed by BAL-31 nuclease digestion of sequences in the 5′ flanking region of this H4 histone gene and were assayed 1n anin vitrotranscription system. No regions upstream from the TATA box were required for site specific initiationin vitro. Data are presented which suggest that sequences located downstream from the 3′ end of the coding region may influence thein vitrotranscription of this human H4 histone gene.