Protein adsorption equilibria and kinetics to a poly(vinyl alcohol)-based magnetic affinity support.
Protein adsorption equilibria and kinetics to a poly(vinyl alcohol)-based magnetic affinity support.
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DOI：
10.1016/s0021-9673(01)00860-3
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发表时间：
2001-07
期刊：
影响因子：
--
通讯作者：
B. Xue;Y. Sun
中科院分区：
文献类型：
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作者：
B. Xue;Y. Sun
采用乳化交联法制备了一种以聚乙烯醇为基的包裹fe3o4胶体的磁性凝胶。用Cibacron蓝3GA对凝胶进行修饰，形成了一种磁性亲和载体。吸附平衡研究表明，溶菌酶的吸附等温线接近矩形，容量为254 mg/ml，而牛血清白蛋白的吸附等温线符合亨利定律。采用孔隙扩散模型和均匀扩散模型对两种蛋白的摄取动力学进行了研究和分析。实验结果表明，该磁性亲和凝胶具有良好的磁性响应性和蛋白质吸附性能，具有良好的机械和化学稳定性。
A poly(vinyl alcohol)-based magnetic gel entrapping Fe3O4colloids has been prepared by an emulsification-crosslinking method. The gel was modified with Cibacron blue 3GA, and thus a magnetic affinity support was produced. The adsorption equilibrium studies showed that the adsorption isotherm of lysozyme was nearly rectangular, with a capacity of 254 mg/ml, while the adsorption isotherm of bovine serum albumin obeyed the Henry’s law. Uptake kinetics of the two proteins was investigated and analyzed with a pore diffusion model and a homogeneous diffusion model. Experimental results showed that the magnetic affinity gel had magnetic responsiveness and favorable properties in protein adsorption, and was mechanically and chemically stable.