Effect of interleukin-18 on metastasis of mouse osteosarcoma cells
Effect of interleukin-18 on metastasis of mouse osteosarcoma cells
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DOI：
10.1007/s00262-005-0097-3
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发表时间：
2006-01
期刊：
影响因子：
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通讯作者：
Yoshiteru Nakamura;N. Yamada;H. Ohyama;K. Nakasho;Y. Nishizawa;T. Okamoto;H. Futani;S. Yoshiya;H. Okamura;N. Terada
中科院分区：
文献类型：
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作者：
Yoshiteru Nakamura;N. Yamada;H. Ohyama;K. Nakasho;Y. Nishizawa;T. Okamoto;H. Futani;S. Yoshiya;H. Okamura;N. Terada
用抗去唾液酸GM 1血清处理裸鼠，以排除IL-18通过激活T细胞和自然杀伤细胞的抗肿瘤作用，研究IL-18对高转移性LM 8小鼠骨肉瘤细胞转移的影响。将不表达IL-18受体β的LM 8细胞注射到尾静脉中导致肺和肝转移灶的形成。从细胞注射的第五天开始每天注射IL-18对转移灶的数量没有显著影响，而在细胞注射之前和之后每天注射五次IL-18导致显著减少。LM 8细胞与IL-18一起培养5天，然后注射到小鼠体内，对肺和肝转移灶的数量没有显著影响。相比之下，在细胞注射前用IL-18预处理小鼠5天显著减少转移灶。LM 8细胞在其注射后24小时在肺中的保留也通过用IL-18预处理小鼠而减少。从用IL-18预处理5天的小鼠获得的血清抑制LM 8细胞的移动性，但IL-18本身不抑制。这些结果表明，IL-18部分地通过在宿主中诱导抑制细胞移动性的因子来抑制LM 8细胞的转移。
The effect of interleukin-18 (IL-18) on metastasis of highly metastatic LM8 mouse osteosarcoma cells was investigated using nude mice treated with anti-asialo GM1 serum to exclude anti-tumor actions of IL-18 through activation of T and natural killer cells. Injection of LM8 cells which do not express IL-18 receptor β into a tail vain resulted in the formation of pulmonary and hepatic metastatic foci. Daily injection of mice with IL-18 starting the fifth day from the cell injection had no significant effect on the number of metastatic foci, while five daily injections of IL-18 before and after the cell injection resulted in marked decreases. Culture of LM8 cells with IL-18 for 5 days before the injection into mice produced no significant effect on the number of pulmonary and hepatic metastatic foci. In contrast, pretreatment of mice with IL-18 for 5 days before the cell injection markedly decreased metastatic foci. The retention of LM8 cells in the lung 24 h after their injection was also reduced by the pretreatment of mice with IL-18. Serum obtained from mice pretreated with IL-18 for 5 days suppressed mobility of LM8 cells but IL-18 itself did not. These results suggest that IL-18 inhibits metastasis of LM8 cells partly by inducing a factor(s) in the host which suppresses cell mobility.