DISSOCIATION OF GROWTH AND FUNCTION IN THE RAT-THYROID DURING PROLONGED GOITROGEN ADMINISTRATION
DISSOCIATION OF GROWTH AND FUNCTION IN THE RAT-THYROID DURING PROLONGED GOITROGEN ADMINISTRATION
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DOI：
10.1530/acta.0.1010210
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
WILLIAMS, ED
中科院分区：
文献类型：
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作者：
WYNFORDTHOMAS, D;STRINGER, BMJ;WILLIAMS, ED
本研究旨在探讨在生长和功能的变化，发生在大鼠甲状腺在长时间的促甲状腺激素刺激。在5个月的时间内，以频繁的间隔将维持在致甲状腺肿剂氨基三唑上的动物与对照一起处死。用放射免疫法测定血清T3、T4和TSH水平。通过测量甲状腺/血清碘比值（T/S）评估功能活动，通过测量甲状腺重量、滤泡细胞数量和滤泡细胞有丝分裂活性评估生长。血清T3和T4迅速下降到检测不到的水平在2周内。血清TSH水平上升到一个稳定的5倍的最大值后4周。T/S比遵循非常相似的模式，上升到持续的7倍最大值。甲状腺重量和滤泡细胞数量在最初几周迅速增加，但生长速度逐渐下降，80天后几乎降至零。有丝分裂活性在7天后急剧上升至30倍的峰值，但在80天后几乎下降至正常水平，这与观察到的细胞数量变化一致。因此，结果表明，甲状腺滤泡细胞的功能和增殖活性之间的明确分离，在长期刺激血清TSH的持续升高，并指出存在特定的生长调节机制，限制有丝分裂反应。
This study was designed to investigate the changes in growth and function which occur in the rat thyroid during prolonged TSH stimulation. Animals maintained on the goitrogen aminotriazole were sacrificed together with controls at frequent intervals over a period of 5 mo. The levels of serum T3 [triiodothyronine] and T4 [thyroxine] and TSH were measured by radioimmunoassay. Functional activity was assessed by measurement of the thyroid/serum iodide ratio (T/S) and growth by measurement of thyroid weight, follicular cell number and follicular cell mitotic activity. Serum T3 and T4 rapidly fell to undetectable levels within 2 wk. The level of serum TSH rose to a stable 5-fold maximum after 4 wk. The T/S ratio followed a closely similar pattern rising to a sustained 7-fold maximum. Thyroid weight and follicular cell number increased rapidly for the first few weeks but the growth rate declined progressively, falling almost to zero after 80 days. Mitotic activity rose dramatically to a 30-fold peak after 7 days but then declined almost to normal after 80 days, consistent with the observed change in cell number. The results thus demonstrate a clear dissociation between the functional and proliferative activity of the thyroid follicular cells during prolonged stimulation by a sustained elevation of serum TSH and point to the existence of specific growth regulating mechanisms which limit the mitotic response.