Importance of glutathione for growth and survival of Escherichia coli cells:: Detoxification of methylglyoxal and maintenance of intracellular K+
Importance of glutathione for growth and survival of Escherichia coli cells:: Detoxification of methylglyoxal and maintenance of intracellular K+
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DOI：
10.1128/jb.180.16.4314-4318.1998
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发表时间：
1998-08-01
影响因子：
3.2
通讯作者：
Booth, IR
中科院分区：
文献类型：
--
作者：
Ferguson, GP;Booth, IR
研究了谷胱甘肽三肽在大肠杆菌细胞生长和存活中的作用。谷胱甘肽缺乏的突变体泄漏钾并降低细胞质pH。这些突变体比亲本菌株对甲基乙二醛更敏感，这表明在没有谷胱甘肽依赖的解毒作用的情况下，细胞质的酸化不能完全保护细胞。然而，提高谷胱甘肽缺乏菌株的细胞内pH会导致对甲基乙二醛的敏感性增强。这表明，在缺乏谷胱甘肽的情况下，细胞质的酸化可以为大肠杆菌细胞提供一些保护。在KDP系统存在的情况下，谷胱甘肽缺乏突变株对甲基乙二醛高度敏感。这是由于这些细胞内的pH值较高。在没有甲基乙二醛的情况下，谷胱甘肽缺乏菌株中KDP系统的存在也导致在稀释到新鲜介质时存在较长的滞后。这些数据突显了谷胱甘肽对于调节KF池和暴露于甲基乙二醛的生存的重要性。
The role of the tripeptide glutathione in the growth and survival of Escherichia coli cells has been investigated. Glutathione-deficient mutants leak potassium and have a reduced cytoplasmic pH. These mutants are more sensitive to methylglyoxal th an the parent strain, indicating that in the absence of glutathione-dependent detoxification, acidification of the cytoplasm cannot fully protect cells. However, increasing the intracellular pH of the glutathione-deficient strain resulted in enhanced sensitivity to methylglyoxal. This suggests that acidification of the cytoplasm can provide some protection to E. coli cells in the absence of glutathione. In the presence of the Kdp system, glutathione-deficient mutants are highly sensitive to methylglyoxal. This is due to the higher intracellular pH in these cells. In the absence of methylglyoxal, the presence of the Kdp system in a glutathione-deficient strain also leads to an extended lag upon dilution into fresh medium. These data highlight the importance of glutathione for the regulation of the Kf pool and survival of exposure to methylglyoxal.