MAMMALIAN CELL TRANSFORMATIONS IN-VITRO .2. FURTHER CHANGES IN DIFFERENTIATION STATE ACCOMPANYING CONVERSION OF CHINESE HAMSTER CELLS TO FIBROBLASTIC FORM BY DIBUTYRYL ADENOSINE CYCLIC 3',5'-MONOPHOSPHATE AND HORMONES - (CONTACT INHIBITION/COLLAGEN SYNTHESIS/AGGLUTINATION)
MAMMALIAN CELL TRANSFORMATIONS IN-VITRO .2. FURTHER CHANGES IN DIFFERENTIATION STATE ACCOMPANYING CONVERSION OF CHINESE HAMSTER CELLS TO FIBROBLASTIC FORM BY DIBUTYRYL ADENOSINE CYCLIC 3',5'-MONOPHOSPHATE AND HORMONES - (CONTACT INHIBITION/COLLAGEN SYNTHESIS/AGGLUTINATION)
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DOI：
10.1073/pnas.68.7.1648
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发表时间：
1971-01-01
影响因子：
11.1
通讯作者：
PUCK, TT
中科院分区：
文献类型：
--
作者：
HSIE, AW;JONES, C;PUCK, TT
用相对大量的二丁酰腺苷环3′：5′-单磷酸或少量的二丁酰腺苷环3′：5′-单磷酸与睾酮联合作用，可使体外培养的中国仓鼠卵巢细胞发生形态学上的转化，并出现下列附加特性：获得严格的接触生长抑制，使细胞的随机生长模式重新定向为与其长边平行生长的模式;细胞周围随机分布的、节状伪足结构消失;诱导胶原合成;以及被植物凝集素和特异性细胞抗体凝集和聚集的能力降低。这些特征的变化与从恶性到正常成纤维细胞状态的转化一致。这种转换是在遗传控制下，如具有改变的特性的特定突变体的产生所证明的。对睾酮的反应是特异性的，因为雌二醇和氢化可的松等类固醇是无活性的，其他类固醇的活性有限。一些野牡丹素的活性与睾酮和5α-二氢睾酮相等。该系统在哺乳动物细胞表型表达调控的研究中似乎是有用的。
The morphological conversionin vitroof Chinese hamster ovary cells to a fibroblast form by a relatively large amount of dibutyryl adenosine cyclic 3′:5′-monophosphate, or by a combination of small amounts of this compound and testosterone, is attended by appearance of the following additional properties: acquisition of strict contact inhibition of growth; reorientation of the random growth pattern into one in which cells grow parallel to their long dimension; disappearance of the randomly distributed, knob-like, pseudopodal structures around the cell periphery; induction of collagen synthesis; and decrease in the ability to be agglutinated and rounded up by plant agglutinins and specific cell antibodies. The changes in these characteristics are consistent with the conversion from a malignant to a normal fibroblastic state. This conversion is under genetic control, as demonstrated by the production of specific mutants with altered characteristics. The response to testosterone is specific since steroids like estradiol and hydrocortisone are inactive, and others have limited activity. Some prostaglandins are equal in activity to testosterone and 5α-dihydrotestosterone. This system appears useful in study of the regulation of phenotypic expression in mammalian cells.