Diffraction-unlimited three-dimensional optical nanoscopy with opposing lenses
Diffraction-unlimited three-dimensional optical nanoscopy with opposing lenses
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DOI：
10.1038/nphoton.2009.112
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发表时间：
2009-07-01
期刊：
影响因子：
35
通讯作者：
Egner, Alexander
中科院分区：
文献类型：
--
作者：
Hell, Stefan W.;Schmidt, Roman;Egner, Alexander
远场光学显微镜的分辨率停滞了一个世纪，但在20世纪90年代开始探索，导致透明荧光物体的三维纳米成像。在这一追求中的重要因素是两个相对透镜的孔径的合成和相邻标记物的荧光的调制或切换。第一个元件通过将轴向分辨率提高三到七倍而提供了几乎各向同性的三维分辨率，第二个元件使衍射障碍得以克服。在这里，我们回顾了最近的进展，这两个元素的协同组合，非侵入性地提供了一个各向同性的衍射无限的三维分辨率在透明的物体。
The resolution of far-field optical microscopy stagnated for a century, but a quest began in the 1990s leading to nanoscale imaging of transparent fluorescent objects in three dimensions. Important elements in this pursuit were the synthesis of the aperture of two opposing lenses and the modulation or switching of the fluorescence of adjacent markers. The first element provided nearly isotropic three-dimensional resolution by improving the axial resolution by three-to sevenfold, and the second enabled the diffraction barrier to be overcome. Here, we review recent progress in the synergistic combination of these two elements which non-invasively provide an isotropic diffraction-unlimited three-dimensional resolution in transparent objects.