Nanoparticle sizing method based on fluorescence anisotropy analysis
Nanoparticle sizing method based on fluorescence anisotropy analysis
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基于荧光各向异性分析的纳米粒子尺寸测定方法
DOI：
10.1016/j.measurement.2014.08.048
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发表时间：
2015
期刊：
影响因子：
5.6
通讯作者：
Shin-Ichi Tanaka
中科院分区：
文献类型：
--
作者：
Terutake Hayashi;Yuki Ishizaki;Masaki Michihata;Yasuhiro Takaya;Shin-Ichi Tanaka
纳米粒子在电气建筑中的应用需求一直在增加。纳米颗粒为改善电路响应提供了新的机会。我们讨论了一种基于荧光各向异性分析评估纳米颗粒尺寸的新方法。通过测量对颗粒尺寸敏感的旋转扩散系数可以评估颗粒尺寸。我们开发了一种通过使用荧光探针标记颗粒来测量旋转扩散系数的系统。我们报告了验证所提出方法可行性的基础实验。使用所提出的方法测量了直径范围为 6-20 nm 的金纳米粒子的旋转扩散系数。测量的旋转扩散系数随着颗粒尺寸的增加而减小。这一发现表明小于 15 nm 的纳米粒子可以以高分辨率进行测量。
Demand for applications of nanoparticles in electric architecture has been increasing. Nanoparticles provide new opportunities for improving circuit response. We discuss a novel method for evaluating nanoparticle sizes based on fluorescence anisotropy analysis. Particle size evaluation is possible through measurements of the rotational diffusion coefficient, which is sensitive to particle size. We develop a system for measuring rotational diffusion coefficients by using a fluorescent probe to label a particle. We report fundamental experiments that verify the feasibility of the proposed method. The rotational diffusion coefficients of gold nanoparticles with diameters ranging 6–20 nm were measured using the proposed method. The measured rotational diffusion coefficients decrease with increasing particle size. This finding indicates that nanoparticles smaller than 15 nm can be measured with fine resolution.