Enhanced Cell Density Culture of Thiobacillus ferrooxidans in Membrane-Type Bioreactor with Electrolytic Reduction Unit for Ferric Ion
Enhanced Cell Density Culture of Thiobacillus ferrooxidans in Membrane-Type Bioreactor with Electrolytic Reduction Unit for Ferric Ion
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DOI：
10.1252/jcej.24.291
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发表时间：
1991
影响因子：
0.8
通讯作者：
M. Taya;H. Shiraishi;Tetsuya Katsunishi;S. Tone
中科院分区：
文献类型：
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作者：
M. Taya;H. Shiraishi;Tetsuya Katsunishi;S. Tone
利用带有细胞过滤和铁回收单元的膜式生物反应器，实现了氧化亚铁硫杆菌的高密度培养。在阴离子交换膜电解槽中，微生物产生的Fe3+可以在不损失主要营养物质的情况下还原为Fe2+。结果表明，氧化亚铁假单胞菌在电化学再生培养基中生长活跃。在重复分批培养中，细胞过滤和再生培养基的补料，获得的细胞浓度至少是常规分批培养的4-11倍。在这个培养系统中，通过测量培养基氧化还原电位，可以估计细胞和铁的浓度，并确定培养物过滤和培养基喂饲操作的时间。
Enhanced cell density culture of Thiobacillus ferrooxidans was achieved by using a membrane-type bioreactor with cell filtration and iron recycling units. Using an electrolytic bath with an anion exchange membrane, microbially produced Fe3+ could be reduced to Fe2+ without loss of main nutrients in the medium. It was confirmed that T. ferrooxidans exhibited active growth in the electrochemically regenerated medium. In the repeated-batch culture with cell filtration and feeding of the regenerated medium, the cell concentration obtained was at least 4–11 times higher than that in a conventional batch culture. In this culture system, the measurement of medium redox potential made it possible to estimate the concentrations of cells and iron, and to determine the time for culture filtration and medium-feeding operations.