Mesenchymal Stem Cells Differentiation on Hierarchically Micro/Nano-Structured Titanium Substrates
Mesenchymal Stem Cells Differentiation on Hierarchically Micro/Nano-Structured Titanium Substrates
复制标题
分层微/纳米结构钛基底上的间充质干细胞分化
DOI：
10.1002/adem.201180073
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发表时间：
2012-05-01
影响因子：
3.6
通讯作者：
Tang, Liling
中科院分区：
文献类型：
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作者：
Chen, Xiuyong;Cai, Kaiyong;Tang, Liling
生物材料的表面形貌在调节细胞的黏附、增殖和分化方面起着至关重要的作用。为了研究微/纳米结构的层次化衬底对间充质干细胞分化的影响，采用双重酸蚀和/或阳极氧化的方法制备了一系列微米、纳米和微/纳米结构的钛衬底。分别用场发射扫描电子显微镜(FE-SEM)和接触角测量仪对钛衬底的形貌进行了表征。体外研究MSCs在不同基质上的分化情况。与天然钛相比，微/纳米结构钛基质促进了碱性磷酸酶(ALP)的产生和MSCs的矿化。从骨钙素(OCN)和骨桥蛋白(OPN)的表达可以看出，微纳米结构的钛基质具有诱导MSCs分化的巨大潜力。这项研究提供了一种潜在的替代方案，以产生层次化的微/纳米结构钛基种植体，以增强骨整合。
Surface topography of a biomaterial plays an essential role in the regulation of cell adhesion, proliferation, and differentiation. To investigate the influence of hierarchically micro/nano-structured substrates on the differentiation of mesenchymal stem cells (MSCs), a series of micro-, nano-, and micro/nano-structured titanium substrates were fabricated via dual acid etching and/or anodic oxidation. The morphologies of titanium substrates were characterized by field emission scanning electron microscopy (FE-SEM) and contact angle measurement, respectively. The differentiation of MSCs adhered to different substrates was investigated in vitro. Compared to native titanium, micro/nano-structured titanium substrates improved the alkaline phosphatase (ALP) production and mineralization of MSCs. More importantly, micro/nano-structured titanium substrates demonstrated great potential to induce the differentiation of MSCs, which was revealed by the expressions of osteocalcin (OCN) and osteopontin (OPN). The study provides a potential alternative to generate hierarchically micro/nano-structured titanium-based implant for enhanced osseointegration.