Integrated and Automated, Sample-In to Result-Out, System for Nanotechnology-Based Detection of Infectious Pathogens
Integrated and Automated, Sample-In to Result-Out, System for Nanotechnology-Based Detection of Infectious Pathogens
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用于基于纳米技术的传染性病原体检测的集成和自动化、从样本输入到结果输出的系统
DOI：
10.1166/nnl.2018.2802
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发表时间：
2018-10-01
影响因子：
--
通讯作者：
He, Nongyue
中科院分区：
文献类型：
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作者：
Chen, Hui;Wu, Yanqi;He, Nongyue
设计了一种基于纳米技术的核酸检测系统，用于实时鉴定来自粪便、痰液和血清等不同类型样品的病原体。为了评估该系统的性能，将生成的结果与江苏省疾病预防控制中心工作人员在受控环境下使用天龙自动提取和LightCycler检测对同一样品进行的检测结果进行比较。结果表明，两种检测平台对不同的样品类型和不同的病毒产生了基本相同的实时荧光曲线。与天龙自动提取和LightCycler检测分别进行核酸提取和实时荧光PCR相比，集成和自动化的封闭检测盒系统在封闭的盒子中进行整个检测过程，避免了任何污染的机会，确保了人员的安全。
An integrated and automated, sample-in to result-out, system for nanotechnology-based nucleic acid detection, was designed and used for real-time identification of pathogens from different types of samples, including feces, sputum, and serum. In order to assess the performance of this novel system, generated results were compared with the results of the assays on the same samples, conducted by the staff of Jiangsu Provincial Center for Disease Control and Prevention (CDC) under a controlled environment, using Tianlong automated extraction and LightCycler detection. The results have documented that the two detection platforms generated essentially identical real-time fluorescence curves for different sample types and different viruses. Compared with the Tianlong automated extraction and LightCycler detection, which separately perform the nucleic acid extraction and real-time fluorescence PCR, the integrated and automated system with closed cartridge carries out the entire detection procedure in a closed box, avoiding any chances of contamination and ensuring the safety of the personnel.