Soil Fungal:Bacterial Ratios Are Linked to Altered Carbon Cycling.
Soil Fungal:Bacterial Ratios Are Linked to Altered Carbon Cycling.
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DOI：
10.3389/fmicb.2016.01247
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发表时间：
2016
影响因子：
5.2
通讯作者：
Gleixner G
中科院分区：
文献类型：
--
作者：
Malik AA;Chowdhury S;Schlager V;Oliver A;Puissant J;Vazquez PG;Jehmlich N;von Bergen M;Griffiths RI;Gleixner G
尽管有几条观察证据，但对于较高的真菌与细菌(F：B)比率是否直接导致土壤碳(C)储量较高，仍缺乏共识。我们使用了RNA测序、蛋白质图谱和同位素示踪技术来评估不同的F：B比率是否与C储存的差异有关。在理化性质相似但微生物群落结构不同的两种土壤上进行了Mesocosm 13C标记的叶凋落物分解实验，特别是F：B比(通过PLFA分析、RNA测序和蛋白质谱测定，三者都是相互印证的)。在添加凋落物后，我们观察到真菌门的丰度持续增加；真菌在以土壤为主的土壤中增加得更多，这意味着真菌在凋落物分解中的作用。在高F：B土壤中，呼吸CO2中的凋落物中的13C含量一直较低，而土壤有机质中残留的13C含量较高，表明F：B占优势的土壤具有较大的碳储存潜力。我们的结论是，在这个土壤系统中，两种土壤中真菌丰度的增加和F：B为主土壤中C循环模式的改变突出了真菌在凋落物分解中的重要作用，并表明F：B比与更高的C储存潜力有关。
Despite several lines of observational evidence, there is a lack of consensus on whether higher fungal:bacterial (F:B) ratios directly cause higher soil carbon (C) storage. We employed RNA sequencing, protein profiling and isotope tracer techniques to evaluate whether differing F:B ratios are associated with differences in C storage. A mesocosm 13C labeled foliar litter decomposition experiment was performed in two soils that were similar in their physico-chemical properties but differed in microbial community structure, specifically their F:B ratio (determined by PLFA analyses, RNA sequencing and protein profiling; all three corroborating each other). Following litter addition, we observed a consistent increase in abundance of fungal phyla; and greater increases in the fungal dominated soil; implicating the role of fungi in litter decomposition. Litter derived 13C in respired CO2 was consistently lower, and residual 13C in bulk SOM was higher in high F:B soil demonstrating greater C storage potential in the F:B dominated soil. We conclude that in this soil system, the increased abundance of fungi in both soils and the altered C cycling patterns in the F:B dominated soils highlight the significant role of fungi in litter decomposition and indicate that F:B ratios are linked to higher C storage potential.