Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols
Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols
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DOI：
10.1177/002215540405200312
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发表时间：
2004-03-01
影响因子：
3.2
通讯作者：
Filgueira, L
中科院分区：
文献类型：
--
作者：
Filgueira, L
破骨细胞是唯一的骨吸收细胞。除了其他特定性质外，破骨细胞的特征在于其抗酒石酸酸性磷酸酶（TRAP）的表达，其通常使用组织化学方法进行光学显微镜检测。利用ELF97磷酸酶底物，本研究描述了一种新的基于荧光的TRAP检测方法。基于荧光的ELF97 TRAP染色不仅可以更好地分辨TRAP阳性颗粒，因为共聚焦显微镜可以用于图像采集和分析，而且它还可以揭示有关破骨细胞的额外和更具体的信息，因为它可以与其他基于荧光的方法相结合。
Osteoclasts are the only bone-resorbing cells. In addition to other specific properties, osteoclasts are characterized by their expression of tartrate-resistant acidic phosphatase (TRAP), which is usually detected using a histochemical method for light microscopy. Using ELF97 phosphatase substrate, this study describes a new fluorescence-based method for TRAP detection. The fluorescence-based ELF97 TRAP stain not only results in a better resolution of the TRAP-positive granules, because confocal microscopy can be applied for image acquisition and analysis, but it reveals additional and more specific information about osteoclasts because it can be combined with other fluorescence-based methods.