Homoeologous recombination-based transfer and molecular cytogenetic mapping of powdery mildew-resistant gene Pm57 from Aegilops searsii into wheat
Homoeologous recombination-based transfer and molecular cytogenetic mapping of powdery mildew-resistant gene Pm57 from Aegilops searsii into wheat
复制标题
西氏山羊草抗白粉病基因 Pm57 向小麦的同源重组转移和分子细胞遗传学作图
DOI：
10.1007/s00122-017-2855-y
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发表时间：
2017-04-01
影响因子：
5.4
通讯作者：
Gill, Bikram S.
中科院分区：
文献类型：
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作者：
Liu, Wenxuan;Koo, Dal-Hoe;Gill, Bikram S.
Pm57 是一种新型抗白粉病基因，从白伊伊伊氏菌（Ae）转入普通小麦中。 searsi 并进一步以 FL0.75 至 FL0.87 的间隔映射到 2Ss#1L。白粉病，由真菌 Blumeria graminis f 引起。 sp。小麦病害是小麦最严重的叶部病害之一，会导致谷物产量和品质下降。宿主植物抗性是控制这种疾病最有效且对环境安全的方法。一组中国Spring-Ae的测试。 searsii (SsSs, 2n = 2x = 14) Feldman & Kislev ex K. Hammer 二体附加系与白粉病真菌的混合分离株鉴定出一种新的抗性基因，设计为 Pm57，位于染色体 2Ss#1 上。在此，我们报道了十种小麦-Ae的开发情况。西尔斯重组体。通过 FISH 和 SSR 标记分析鉴定了其中 5 个重组体涉及的小麦染色体，其中 3 个对白粉病具有抗性。 Pm57 进一步定位到染色体 2Ss#1 的长臂，分数长度间隔为 FL 0.75 至 FL 0.87。具有 28% 远端 2Ss#1L 片段的重组原种 T2BS.2BL-2Ss#1L 89-346 (TA5108) 和具有 33% 的 89(5)69 (TA5109) 可能可用于小麦改良。 PCR 标记 X2L4g9p4/HaeIII 经验证可特异性识别 Ae。针对 16 个小麦品种和先进育种系，T2BS.2BL-2Ss#1L 中含有 Pm57 的 searsii 2Ss#1L 片段，并且正在开发更多用户友好的 KASP 标记。
Pm57, a novel resistant gene against powdery mildew, was transferred into common wheat from Ae. searsi and further mapped to 2Ss#1L at an interval of FL0.75 to FL0.87. Powdery mildew, caused by the fungus Blumeria graminis f. sp. tritici, is one of the most severe foliar diseases of wheat causing reduction in grain yield and quality. Host plant resistance is the most effective and environmentally safe approach to control this disease. Tests of a set of Chinese Spring-Ae. searsii (SsSs, 2n = 2x = 14) Feldman & Kislev ex K. Hammer disomic addition lines with a mixed isolate of the powdery mildew fungus identified a novel resistance gene(s), designed as Pm57, which was located on chromosome 2Ss#1. Here, we report the development of ten wheat-Ae. searsii recombinants. The wheat chromosomes involved in five of these recombinants were identified by FISH and SSR marker analysis and three of them were resistant to powdery mildew. Pm57 was further mapped to the long arm of chromosome 2Ss#1 at a fraction length interval of FL 0.75 to FL 0.87. The recombinant stocks T2BS.2BL-2Ss#1L 89-346 (TA5108) with distal 2Ss#1L segments of 28% and 89(5)69 (TA5109) with 33% may be useful in wheat improvement. The PCR marker X2L4g9p4/HaeIII was validated to specifically identify the Ae. searsii 2Ss#1L segment harboring Pm57 in T2BS.2BL-2Ss#1L against 16 wheat varieties and advanced breeding lines, and the development of more user-friendly KASP markers is underway.