Tissue damage after single high-dose intraoperative irradiation of the canine liver: Evaluation in time by means of radionuclide imaging and light microscopy
Tissue damage after single high-dose intraoperative irradiation of the canine liver: Evaluation in time by means of radionuclide imaging and light microscopy
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DOI：
10.1667/0033-7587(2000)154
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发表时间：
2000-11-01
影响因子：
3.4
通讯作者：
Hoekstra, HJ
中科院分区：
文献类型：
--
作者：
Cromheecke, M;Piers, BA;Hoekstra, HJ
为探讨肝组织对术中单次大剂量照射的耐受性，采用放射性核素显像和光镜技术，观察了25只比格犬的部分肝脏在术中单次大剂量照射后的组织病理学变化。在随访期间多次使用Tc-99 m-硫胶体进行放射性核素显像。分别于照射后3个月、1年、2年、3年和5年进行选择性人道处死，光镜观察组织病理学变化。在最长5年的随访期间没有发病率或死亡率。在40%的动物中，在照射部位观察到摄取减少的区域，放射性药剂摄取减少的区域随着时间的推移而变小。光镜检查显示，严重的实质纤维化，肝细胞萎缩，胆管增生的照射区照射后1至2年。在3年和5年时，观察到血管变化，伴有内皮增生和局灶性小动脉玻璃样变性。本研究表明，在犬模型中，术中照射部分肝脏是安全的。光学显微镜检查证实，组织学损伤并不总是伴随着放射性药物在照射部位的摄取减少。(C)2000年，辐射研究协会。
To establish the tolerance of liver tissue to single high-dose intraoperative irradiation, the histopathological changes in the canine liver after single high-dose intraoperative irradiation were investigated by means of radionuclide imaging and light microscopy, Intraoperative irradiation at doses of 0, 10, 20, 25 or 30 Gy was applied to a part of the liver of 25 beagles. Radionuclide imaging using Tc-99m-sulfur colloid was performed at several times during follow-up. Elective humane killing was done 3 months and 1, 2, 3 and 5 years after irradiation, Light microscopy was used to identify histopathological alterations. There was no morbidity or mortality during a maximal followup of 5 years. In 40% of the animals, a region of diminished uptake was observed at the irradiation site, The regions of diminished uptake of the radiopharmaceutical agent became smaller with time. Light microscopic examination revealed severe parenchymal fibrosis, liver cell atrophy, and bile duct proliferation at the irradiated area 1 to 2 years after irradiation. At 3 and 5 years, vascular changes with endothelial proliferation and focal arteriolar hyalinosis were observed, This study demonstrates that intraoperative irradiation of a part of the liver in the canine model can be applied safely. Light microscopy confirmed that histological damage was not always accompanied by diminished uptake of the radiopharmaceutical agent at the irradiation site. (C) 2000 by Radiation Research Society.