An in vivo evaluation of a biodegradable genipin-cross-linked gelatin peripheral nerve guide conduit material
An in vivo evaluation of a biodegradable genipin-cross-linked gelatin peripheral nerve guide conduit material
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DOI：
10.1016/j.biomaterials.2004.09.060
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发表时间：
2005-06-01
期刊：
影响因子：
14
通讯作者：
Liu, BS
中科院分区：
文献类型：
--
作者：
Chen, YS;Chang, JY;Liu, BS
我们评估周围神经再生使用可生物降解的神经导管，这是京尼平交联明胶制成。京尼平交联明胶导管（GGC）外观为深蓝色，呈同心圆状，外表面粗糙，内腔光滑。在大鼠背侧皮下植入后，GGC仅引起轻微的组织反应，在导管周围形成薄的组织囊。当GGC用于修复大鼠坐骨神经中10 mm的间隙时，检查了GGC的生物降解性及其作为引导通道的有效性。结果表明，移植后6周，导管断裂不明显，在4、6、8周三个实验阶段，所有导管均通过差距成功再生。组织学观察显示，术后6周有大量再生神经纤维穿过并越过差距区，这些纤维多为无髓纤维，周围有雪旺细胞包裹。肌肉动作电位的峰幅和曲线下面积均随恢复时间的延长而增加，表明神经已充分再生。因此，GGC不仅可以是再生神经的有效辅助物，而且还可以导致有利的神经功能恢复。(C)2004 Elsevier Ltd.保留所有权利。
We evaluated peripheral nerve regeneration using a biodegradable nerve conduit, which was made of genipin-cross-linked gelatin. The genipin-cross-linked gelatin conduit (GGC) was dark blue in appearance, which was concentric and round with a rough outer surface whereas its inner lumen was smooth. After subcutaneous implantation on the dorsal side of the rat, the GGC only evoked a mild tissue response, forming a thin tissue capsule surrounding the conduit. Biodegradability of the GGC and its effectiveness as a guidance channel were examined as it was used to repair a 10 mm gap in the rat sciatic nerve. As a result, tube fragmentation was not obvious until 6 weeks post-implantation and successful regeneration through the gap occurred in all the conduits at the three experimental periods of 4, 6, and 8 weeks. Histological observation showed that numerous regenerated nerve fibers, mostly unmyelinated and surrounded by Schwann cells, crossed through and beyond the gap region 6 weeks after operation. Peak amplitude and area under the muscle action potential curve both showed an increase as a function of the recovery period, indicating that the nerve had undergone adequate regeneration. Thus, the GGC can not only be an effective aids for regenerating nerves but can also lead to favorable nerve functional recovery. (C) 2004 Elsevier Ltd. All rights reserved.