MicroRNA156 conditions auxin sensitivity to enable growth plasticity in response to environmental changes in Arabidopsis.
MicroRNA156 conditions auxin sensitivity to enable growth plasticity in response to environmental changes in Arabidopsis.
复制标题
DOI：
10.1038/s41467-023-36774-9
复制
发表时间：
2023-03-22
影响因子：
16.6
通讯作者：
Chen, Xuemei
中科院分区：
文献类型：
--
作者：
Sang, Qing;Fan, Lusheng;Liu, Tianxiang;Qiu, Yongjian;Du, Juan;Mo, Beixin;Chen, Meng;Chen, Xuemei
MicroRNAs(MiRNAs)在植物发育中发挥着不同的作用，但miRNAs是否以及如何参与热形态发生仍不清楚。在这里，我们证明了下胚轴生长的可塑性依赖于温度的拟南芥下胚轴生长的可塑性中，下胚轴生长的关键成分HYL1(HYL1)在温度调节光敏色素相互作用因子4的下游作用。一个hyl1-2抑制基因筛选确定了一个显性的Dicer-like 1等位基因，它挽救了hyl1-2‘S在miRNA生物发生和对温度反应的下胚轴伸长中的缺陷。全基因组miRNA和转录组分析表明，microRNA156(MiR156)及其靶基因Squamosa启动子结合蛋白样9(SPL9)是热形态发生的关键调控因子。令人惊讶的是，miR156/SPL9模块的扰动通过阻碍生长素的敏感性来解除幼苗对温暖温度的反应。此外，依赖miR156的生长素敏感性也在较低温度下的遮荫回避反应中发挥作用。因此，这些结果揭示了miR156/SPL9模块是一个以前未被描述的遗传电路，它使植物生长可塑性对环境温度和光照变化做出反应。植物通过光感受器光敏色素B和植物激素生长素来响应光和温度的变化。在这里，作者揭示了microRNA156是一个以前未被描述的发育信号，它通过许可生长素敏感性来控制环境调节的植物生长。
MicroRNAs (miRNAs) play diverse roles in plant development, but whether and how miRNAs participate in thermomorphogenesis remain ambiguous. Here we show that HYPONASTIC LEAVES 1 (HYL1)—a key component of miRNA biogenesis—acts downstream of the thermal regulator PHYTOCHROME INTERACTING FACTOR 4 in the temperature-dependent plasticity of hypocotyl growth in Arabidopsis. A hyl1-2 suppressor screen identified a dominant dicer-like1 allele that rescues hyl1-2’s defects in miRNA biogenesis and thermoresponsive hypocotyl elongation. Genome-wide miRNA and transcriptome analysis revealed microRNA156 (miR156) and its target SQUAMOSA PROMOTER-BINDING-PROTEIN-LIKE 9 (SPL9) to be critical regulators of thermomorphogenesis. Surprisingly, perturbation of the miR156/SPL9 module disengages seedling responsiveness to warm temperatures by impeding auxin sensitivity. Moreover, miR156-dependent auxin sensitivity also operates in the shade avoidance response at lower temperatures. Thus, these results unveil the miR156/SPL9 module as a previously uncharacterized genetic circuit that enables plant growth plasticity in response to environmental temperature and light changes. Plants respond to light and temperature changes via the photoreceptor phytochrome B and the phytohormone auxin. Here the authors reveal microRNA156 as a previously uncharacterized developmental signal that gates environmentally regulated plant growth by licensing auxin sensitivity.
登录
查看更多内容
影响因子：
7.2
作者：
Cheng, Chia-Yi;Krishnakumar, Vivek;Town, Christopher D.
通讯作者：
Town, Christopher D.
影响因子：
9.4
作者：
de Wit, Mieke;Ljung, Karin;Fankhauser, Christian
通讯作者：
Fankhauser, Christian
影响因子：
9.2
作者：
Ibanez, Carla;Delker, Carolin;Quint, Marcel
通讯作者：
Quint, Marcel
影响因子：
7.4
作者：
Bellstaedt, Julia;Trenner, Jana;Delker, Carolin
通讯作者：
Delker, Carolin
影响因子：
7.4
作者：
Golden, TA;Schauer, SE;Ray, A
通讯作者：
Ray, A