FETAL ALCOHOL EFFECTS IN LONG-SLEEP AND SHORT-SLEEP MICE - ACTIVITY, PASSIVE-AVOIDANCE, AND INUTERO ETHANOL LEVELS
FETAL ALCOHOL EFFECTS IN LONG-SLEEP AND SHORT-SLEEP MICE - ACTIVITY, PASSIVE-AVOIDANCE, AND INUTERO ETHANOL LEVELS
复制标题
DOI：
10.1016/0892-0362(87)90030-4
复制
发表时间：
1987-09-01
影响因子：
2.9
通讯作者：
RILEY, EP
中科院分区：
文献类型：
--
作者：
GILLIAM, DM;STILMAN, A;RILEY, EP
人类和动物研究都表明胎儿酒精效应（FAE）易感性的遗传差异。长睡眠（LS）和短睡眠（SS）小鼠系，选择性地培育乙醇诱导的麻醉的差异，提供了一个模型，研究酒精敏感性的差异在FAE的病因。在妊娠第7天至第15天，对LS和SS小鼠进行2.9 g/kg（20%，w/v）乙醇（E）或等热量蔗糖（S）插管，每天两次（间隔6小时）。对于每个系保持未处理的对照组（C）。后代在出生时被寄养在泌乳的Rockland-Swiss小鼠中。出生前暴露于乙醇的LS后代表现出相对于LS对照组的旷场活动增加，但这种影响是由于一窝的过度活动。产前暴露于乙醇的SS小鼠的活性与对照水平没有差异。在相似处理的LS和SS母鼠中，血液（280 mg/dl）、羊水（258 mg/dl）和胎仔组织（230 mg/dl）中的乙醇含量无差异。在第二个实验中，雌性动物在妊娠第7天至第18天接受处理，并对其后代进行旷场活动或被动回避学习测试。在旷场活动方面没有组间差异，但产前暴露于酒精的LS小鼠比对照组进行了更多的试验以达到被动回避标准，而同样处理的SS小鼠与对照组没有差异。这些结果表明，遗传介导的敏感性乙醇影响FAE的易感性，这可能是特定的任务。
Genetic differences in susceptibility to fetal alcohol effects (FAE) have been suggested by both human and animal studies. The Long-Sleep (LS) and Short-Sleep (SS) mouse lines, selectively bred for differences in ethanol-induced narcosis, provide a model for studying differential alcohol sensitivity in the etiology of FAE. LS and SS mice were intubated with either 2.9 g/kg (20%, w/v) ethanol (E) or an isocaloric amount of sucrose (S) twice per day (6 hr apart) on Days 7 through 15 of pregnancy. An untreated control group (C) was maintained for each line. Offspring were fostered to lactating Rockland-Swiss mice at birth. LS offspring prenatally exposed to ethanol exhibited increased open-field activity relative to the LS controls, but this effect was due to the overactivity of one litter. Activity for SS mice prenatally exposed to ethanol did not differ from control levels. Ethanol content in blood (280 mg/dl), amniotic fluid (258 mg/dl), and fetal tissue (230 mg/dl) did not differ in similarly treated LS and SS dams. In a second experiment, female were treated from Days 7 through 18 of gestation, and their offspring were tested for either open-field activity or passive avoidance learning. There were no group differences in open-field activity, but LS mice prenatally exposed to alcohol took more trials to reach a passive avoidance criterion than their controls, whereas similarly treated SS mice did not differ from controls. These results suggest that genetically-mediated sensitivity to ethanol influences susceptibility to FAE and that this may be task specific.