Effects of Jixueteng on Experimental Periodontitis During Orthodontic Tooth Movement in Rats
Effects of Jixueteng on Experimental Periodontitis During Orthodontic Tooth Movement in Rats
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DOI：
10.1177/1934578x211002419
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发表时间：
2021-04
影响因子：
1.8
通讯作者：
T. Katayama;Takenori Sato;N. Hamada;S. Goda;Tetsutaro Yamaguchi;K. Tsukinoki;K. Handa
中科院分区：
文献类型：
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作者：
T. Katayama;Takenori Sato;N. Hamada;S. Goda;Tetsutaro Yamaguchi;K. Tsukinoki;K. Handa
近年来，天然成分在正畸治疗中抑制细菌引起的牙槽骨吸收方面的研究越来越受到重视。中药鸡血藤含有多种黄酮类化合物，具有生物学活性。本实验观察了Jix对大鼠正畸牙移动过程中实验性牙周炎的影响。为此，9周龄的雄性Wistar大鼠，这是配备了正畸矫治器，口腔感染牙龈卟啉单胞菌（Pg），而Jix在其饮用水管理。在OTM开始后共28天，用微型计算机断层扫描对上颌右侧的牙槽骨吸收进行扫描。这些也被用作组织学标本，并进行抗酒石酸酸性磷酸酶（TRAP）染色。TRAP阳性多核细胞计数为破骨细胞。结果，OTM和Pg感染并给予Jix（OTM + Pg + Jix）组的牙齿移动距离与假感染组相同。OTM + Pg + Jix组的骨吸收量和破骨细胞数较OTM + Pg感染组明显减少（P < 0.05）。因此，Jix对OTM几乎没有影响，并抑制Pg引起的牙槽骨破坏。我们认为，在正畸治疗过程中，Jix的管理可以支持牙齿移动，并有助于预防牙周炎。
Recently, natural ingredients have focused on the inhibition of bacteria-induced alveolar bone resorption in orthodontic treatment. Jixueteng (Jix), a Chinese traditional medicine, contains several kinds of flavonoids given their biological properties. We evaluated the effects of Jix on experimental periodontitis during orthodontic tooth movement (OTM) in rats. To this end, 9-week-old male Wistar rats, which were equipped with orthodontic appliance, were orally infected with Porphyromonas gingivalis (Pg), while Jix was administered in their drinking water. A total of 28 days after the beginning of OTM, alveolar bone resorption on the right side of the upper jaws was scanned with micro-computed tomography. These were also used as histological specimens and underwent tartrate-resistant acid phosphatase (TRAP) staining. TRAP-positive multinucleated cells were counted as osteoclasts. As a result, the distance of tooth movement in the OTM and Pg infection with Jix administration (OTM + Pg + Jix) group was the same as that of the sham-infected group. The amount of bone resorption and number of osteoclasts in the OTM + Pg + Jix group was more significantly decreased than that in the OTM and Pg-infected group (P < 0.05). Hence, Jix had little effect on OTM and inhibited Pg-induced alveolar bone destruction. We suggested that the administration of Jix can support tooth movement and contribute to the prevention of periodontitis during orthodontic treatment.