GENETIC-CHARACTERIZATION AND TRANSOVARIAL TRANSMISSION OF A TYPHUS-LIKE RICKETTSIA FOUND IN CAT FLEAS
GENETIC-CHARACTERIZATION AND TRANSOVARIAL TRANSMISSION OF A TYPHUS-LIKE RICKETTSIA FOUND IN CAT FLEAS
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DOI：
10.1073/pnas.89.1.43
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发表时间：
1992-01-01
影响因子：
11.1
通讯作者：
CARL, M
中科院分区：
文献类型：
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作者：
AZAD, AF;SACCI, JB;CARL, M
对表面上苛养微生物的鉴定常常是有问题的。用来自立克次体17-kDa共同蛋白抗原和柠檬酸合酶基因的保守引物，而不是斑点热组190-kDa抗原基因的保守引物，可以通过PCR扩增猫蚤中存在的立克次体样因子（称为ELB因子）的DNA。用类立克次体试剂和伤寒立克次体获得的17-kDa和柠檬酸合酶PCR产物中的Alu I位点是不同的，即使这两种试剂与先前认为对R.伤寒。立克次体样因子的17-kDa PCR产物的一部分的DNA序列与所有已知的立克次体序列显著不同，并且与斑疹伤寒的17-kDa序列比斑点热组立克次体更相似。这种立克次体在猫蚤中罕见的稳定跨卵维持对猫蚤的疾病潜力具有重要意义。
The identification of apparently fastidious microorganisms is often problematic. DNA from a rickettsia-like agent (called the ELB agent) present in cat fleas could be amplified by PCR with conserved primers derived from rickettsial 17-kDa common protein antigen and citrate synthase genes but not spotted fever group 190-kDa antigen gene. Alu I sites in both the 17-kDa and citrate synthase PCR products obtained with the rickettsia-like agent and Rickettsia typhi were different even though both agents reacted with monoclonal antibodies previously thought specific for R. typhi. The DNA sequence of a portion of the 17-kDa PCR product of the rickettsia-like agent differed significantly from all known rickettsial sequences and resembled the 17-kDa sequences of typhus more than spotted fever group rickettsiae. The rare stable transovarial maintenance of this rickettsia in cat fleas has important implications for the disease potential of cat fleas.