NITRIC-OXIDE SYNTHASE-CONTAINING NEURONS IN THE PIG LARGE-INTESTINE - TOPOGRAPHY, MORPHOLOGY, AND VISCEROFUGAL PROJECTIONS
NITRIC-OXIDE SYNTHASE-CONTAINING NEURONS IN THE PIG LARGE-INTESTINE - TOPOGRAPHY, MORPHOLOGY, AND VISCEROFUGAL PROJECTIONS
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DOI：
10.1002/jemt.1070290203
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发表时间：
1994-10-01
影响因子：
2.5
通讯作者：
DEGROODTLASSEEL, MHA
中科院分区：
文献类型：
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作者：
BARBIERS, M;TIMMERMANS, JP;DEGROODTLASSEEL, MHA
用一氧化氮合酶（NOS）免疫细胞化学和烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPHd）组织化学方法，研究了猪大肠内合成一氧化氮（NO）的神经元的分布。NOS免疫反应性和NADPHd染色的整体共定位观察。氮能神经元丰富的肠肌间神经丛和外粘膜下丛的盲肠，结肠和直肠。只有少数氮能胞体被认为是在结肠和盲肠的内粘膜下神经丛，而大量的观察在直肠。三个神经节神经丛内均存在氮能神经纤维。与外纵肌层和粘膜区相反，环形肌层接受密集的氮能神经支配。氮能神经细胞的大小和形状是可变的，和一些显示血管活性肠多肽（VIP）免疫反应性（IR）。逆行追踪研究表明，存在氮能神经元，项目的尾（下）肠系膜神经节。在横结肠、降结肠和直肠的肌间神经丛和外粘膜下神经丛中观察到它们。这些观察结果强烈表明，几个亚群的NO合成神经元，即运动神经元和中间神经元，应区分在猪大肠，从而强调NO作为一种生物活性介质的重要性。(C)1994 Wiley-Liss，Inc.
The distribution of neurons that are capable of synthesizing nitric oxide (NO) has been demonstrated in the porcine large intestine by means of NO synthase (NOS) immunocytochemistry and nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd) histochemistry. An overall colocalization of NOS immunoreactivity and NADPHd staining was observed. Nitrergic neurons were abundant in the myenteric and outer submucous plexus of the caecum, colon, and rectum. Only a few nitrergic perikarya were seen in the inner submucous plexus of the colon and caecum, whereas a substantially larger number was observed in the rectum. Nitrergic nerve fibers were present in the three ganglionic nerve plexuses. Contrary to the outer longitudinal muscle layer and the mucosal region, the circular muscle layer received a dense nitrergic innervation. The nitrergic nerve cells were variable in size and shape, and several displayed vasoactive intestinal polypeptide (VIP) immunoreactivity (IR). Retrograde tracing studies revealed the existence of nitrergic neurons that project to the caudal (inferior) mesenteric ganglion. They were observed in the myenteric and outer submucous plexus of the transverse and descending colon and the rectum. These observations strongly suggest that several subpopulations of NO-synthesizing neurons, namely, motor neurons and interneurons, should be distinguished in the porcine large intestine, thereby emphasizing the importance of NO as a biologically active mediator. (C) 1994 Wiley-Liss, Inc.