Logic nanoparticle beacon triggered by the binding-induced effect of multiple inputs.
Logic nanoparticle beacon triggered by the binding-induced effect of multiple inputs.
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DOI：
10.1021/am5036994
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发表时间：
2014-08
影响因子：
9.5
通讯作者：
Jing Yang;Chen Dong;Yafei Dong;Shi Liu;L. Pan;Cheng Zhang
中科院分区：
文献类型：
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作者：
Jing Yang;Chen Dong;Yafei Dong;Shi Liu;L. Pan;Cheng Zhang
近年来，立足点介导的DNA链置换反应已广泛用于检测分子信号。然而，传统的链置换由于DNA输入之间没有协同信号传导，不足以设计更复杂的纳米器件。在这项工作中，基于AuNP基信标，使用协作“绑定诱导”机制建立了逻辑计算系统，其中构建了五种多输入逻辑门。该系统可以同时识别DNA和蛋白质链霉亲和素。最后，还通过控制编程的共轭 DNA/AuNP 簇来演示逻辑系统的操作。这项研究提供了检测多个输入信号和设计复杂纳米器件的可能性，这些器件可潜在地应用于检测多个分子目标和构建大规模基于 DNA 的计算。
Recently, the toehold-mediated DNA strand displacement reaction has been widely used in detecting molecular signals. However, traditional strand displacement, without cooperative signaling among DNA inputs, is insufficient for the design of more complicated nanodevices. In this work, a logic computing system is established using the cooperative "binding-induced" mechanism, based on the AuNP-based beacons, in which five kinds of multiple-input logic gates have been constructed. This system can recognize DNA and protein streptavidin simultaneously. Finally, the manipulations of the logic system are also demonstrated by controlling programmed conjugate DNA/AuNP clusters. This study provides the possibility of detecting multiple input signals and designing complex nanodevices that can be potentially applied to the detection of multiple molecular targets and the construction of large-scale DNA-based computation.