The role of the T-cell receptor in thymocyte maturation: effects in vivo of anti-receptor antibody.
The role of the T-cell receptor in thymocyte maturation: effects in vivo of anti-receptor antibody.
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T 细胞受体在胸腺细胞成熟中的作用：抗受体抗体的体内作用。
DOI：
10.1073/pnas.83.22.8728
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发表时间：
1986
影响因子：
11.1
通讯作者：
Kappler,J
中科院分区：
文献类型：
--
作者：
McDuffie,M;Born,W;Marrack,P;Kappler,J
T细胞受体识别抗原和主要组织相容性复合体的产物，被认为通过结合胸腺基质细胞上表达的主要组织相容性复合体蛋白来推动胸腺中T细胞的成熟。我们通过给新生动物注射一种抗受体抗体KJ16来测试这一想法，KJ16可以结合大约20%的T细胞，并能够阻断受体功能。在这种抗体存在的情况下，携带KJ16表位的成熟T细胞无法发育。另一方面，尽管抗体可以被证明与皮质胸腺细胞上的受体结合，但它并不能阻止该人群中大部分KJ16+细胞的快速扩张或存活。这些结果与以下假设一致，即大多数皮质胸腺细胞是通过受体非依赖性机制产生的，并且只有一小部分细胞通过依赖于受体-主要组织相容性复合体相互作用的过程成熟。
The T-cell receptor, which recognizes antigen plus a product of the major histocompatibility complex, has been postulated to drive T-cell maturation in the thymus by engaging major histocompatibility complex proteins expressed on thymic stromal cells. We tested this idea by injecting neonatal animals with an anti-receptor antibody, KJ16, that binds to about 20% of T cells and is capable of blocking receptor function. In the presence of this antibody, mature T cells bearing the KJ16 epitope failed to develop. On the other hand, although the antibody could be shown to bind to receptors on cortical thymocytes, it did not prevent the rapid expansion or survival of the bulk of the KJ16+ cells in this population. These results are consistent with the hypothesis that most cortical thymocytes arise by a receptor-independent mechanism and that only a small proportion of these cells mature by a process dependent on receptor-major histocompatibility complex interactions.