PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe
PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe
复制标题
使用 3D DNA 纳米结构报告探针进行无 PCR 比色 DNA 杂交检测
DOI：
10.1021/acsami.7b11994
复制
发表时间：
2017
影响因子：
9.5
通讯作者：
Liu G.
中科院分区：
文献类型：
--
作者：
Yang X.;Wen Y. L.;Wang L. L.;Zhou C. Q.;Li Q.;Xu L.;Li L. Y.;Shi J. Y.;Lal R.;Ren S. Z.;Li J.;Jia N. Q.;Liu G.
在磁珠平台的基础上，研制了一种“类人”乳腺癌基因（BRCA1）DNA的生物传感器。本研究设计了一种四面体结构的报告探针（TSRP），其中四面体的3个顶点用地高辛（Dig）标记，另一个顶点用检测探针标记。TSRP提供了精确的酶加载和良好组织的空间排列，以优化信号放大。该生物传感器的检测限低至10 fM，这比单个DNA探针（100 pM）低至少4个数量级，并且信号增益比使用三个一维（1D）报告探针的分析高2倍。我们可以区分只有1个碱基错配的DNA序列，并且我们的TSRP生物传感器的性能被证明在PCR产物和真实的胎牛血清（FCS）样品中与在缓冲液中一样好。我们相信这项工作为信号放大策略的发展提供了新的途径。
A “sandwich-like” biosensor was developed on the basis of the magnetic bead platform for sensitive detection of breast cancer 1 (BRCA1) DNA. In the present study, a tetrahedron-structured reporter probe (TSRP) was designed, in which 3 vertices of the tetrahedron were labeled with digoxin (Dig), and the other one was labeled with a detection probe. TSRP here provided accurate enzyme loading and well-organized spatial arrangement for optimized signal amplification. The detection limit of this biosensor was as low as 10 fM, which is at least 4 orders of magnitude lower than that of the single DNA probe (100 pM), and the signal gain was 2 times higher than the analysis using three one-dimensional (1D) reporter probes. We could distinguish DNA sequences with only 1 base mismatch, and the performance of our TSRP biosensor was proven to be equally good in both PCR products and real fetal calf serum (FCS) sample as in buffer. We believe this work provided a novel avenue for the development of signal amplification strategies.