Parvularcula marina sp. nov., isolated from surface water of the South China Sea, and emended description of the genus Parvularcula
Parvularcula marina sp. nov., isolated from surface water of the South China Sea, and emended description of the genus Parvularcula
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Parvularcula 码头 sp。
DOI：
10.1099/ijsem.0.003543
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发表时间：
2019
影响因子：
2.8
通讯作者：
Xi-Ying Zhang
中科院分区：
文献类型：
--
作者：
Lin-Lin Sun;Yan-Ru Dang;Yi Li;Qi-Long Qin;Hai-Nan Su;Ping-Yi Li;Xiu-Lan Chen;Yu-Zhong Zhang;Xi-Ying Zhang
从中国南海表层海水中分离到一株革兰氏阴性好氧鞭毛杆状细菌SM 1705 T。该菌株在10-40 ℃和0.5-13.0%（w/v）NaCl下生长。它水解吐温20、40和60，但不水解淀粉或吐温80，也不将硝酸盐还原为亚硝酸盐。基于16 S rRNA基因序列的系统发育分析表明，菌株SM 1705 T属于小球藻属（Parvularcula），与百慕大小球藻（Parvularcula bermudensis）模式株的序列相似性最高（96.0%），并与百慕大小球藻（Parvularcula bermudensis）在小球藻属内形成一个连贯的分支。主要的细胞脂肪酸被鉴定为总和特征8（C18：1！7 c和/或C18：1！6c）、C16：0和C18：0。极性脂质包括三种未鉴别的糖脂和一种未鉴别的脂质。菌株SM 1705 T的主要呼吸醌为Q10。SM 1705 T基因组DNA G+ C含量为59.3mol%。根据本文提出的多相证据，菌株SM 1705 T代表一个新的小球藻属种，并建议将其命名为海洋小球藻Parvularcula marina sp. nov.。模式菌株为SM 1705 T（= KCTC 62795 T = MCCC 1 K 03505 T = CCTCC AB 2018345 T）。Parvularcula属由Cho和Giovannoni提出[1]，目前包括四个有效的已发表种，即Parvularcula bermudensis [1]，Parvularcula lutaonensis[2]，Parvularcula dongshanensis [3]和Parvularcula flava [4]，另外还有一个种，“Parvularcula oceani”[5]，均起源于海洋环境。在对南海海水样品进行细菌多样性调查的过程中，从表层海水样品中分离到一株类似小球藻的菌株，命名为SM 1705 T。在这项研究中，菌株SM 1705 T的分类特征与多相的方法和结果的基础上，它被建议代表一个新的物种属内的Parvularcula。
A Gram-stain-negative, aerobic, flagellated, rod-shaped bacterial strain, SM1705T, was isolated from a surface seawater sample collected from the South China Sea. The strain grew at 10–40 C and with 0.5–13.0%(w/v) NaCl. It hydrolysed Tweens 20, 40 and 60, but did not hydrolyse starch or Tween 80 nor reduce nitrate to nitrite. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1705T was affiliated with the genus Parvularcula, sharing the highest sequence similarity (96.0%) with type strain of Parvularcula bermudensis and forming a coherent branch together with the latter within the clade of Parvularcula. The major cellular fatty acids were identified as summed feature 8 (C18: 1! 7c and/or C18: 1! 6c), C16: 0 and C18: 0. Polar lipids included three unidentified glycolipids and one unidentified lipid. The major respiratory quinone of strain SM1705T was Q10. The genomic DNA G+ Ccontent of strain SM1705T was 59.3 mol%. Based on the polyphasic evidence presented in this paper, strain SM1705T represents a novel Parvularcula species, for which the name Parvularcula marina sp. nov. is proposed. The type strain is SM1705T (= KCTC 62795T= MCCC 1K03505T= CCTCC AB 2018345T).The genus Parvularcula was proposed by Cho and Giovannoni [1] and currently comprises four validly published species, ie Parvularcula bermudensis [1], Parvularcula lutaonensis[2], Parvularcula dongshanensis [3] and Parvularcula flava [4], and one additional species,‘Parvularcula oceani’[5], all originating from marine environments. During an investigation of the bacterial diversity in seawater samples collected from the South China Sea, a Parvularcula-like strain, designated SM1705T, was isolated from a surface seawater sample. In this study, strain SM1705T was taxonomically characterised with a polyphasic approach and on the basis of the results, it is proposed to represent a novel species within the genus Parvularcula.