Use of ddPCR in experimental evolution studies
Use of ddPCR in experimental evolution studies
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DOI：
10.1111/2041-210x.12467
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发表时间：
2016-03-01
影响因子：
6.6
通讯作者：
Becks, Lutz
中科院分区：
文献类型：
--
作者：
Koch, Hanna;Jeschke, Alina;Becks, Lutz
实验进化是进化生物学家的一个重要研究框架，因为它允许直接测试关于适应和多样性的基本理论，这通常需要随着时间的推移跟踪基因型或等位基因。然而，这需要诸如遗传标记、可区分的形态特征或基因分型等工具，这些工具可能是时间和劳动密集型的，并且特别是如果高通量处理是必要的。在这里，我们提出了一种新的方法相结合的多重ddPCR与实验进化跟踪基因型频率在不同的实验人群随着时间的推移。我们发现该方法对于检测和定量感兴趣的靶标而言是精确和准确的，特别是与传统PCR方法相比。使用多重ddPCR跟踪频率可用于广泛的实验进化应用。
Experimental evolution is an important research framework for evolutionary biologists as it allows direct testing of fundamental theories about adaptation and diversity, which often requires the tracking of genotypes or alleles over time. This however requires tools such as genetic markers, distinguishable morphological characters or genotyping, which can be time and labour intensive, and especially if high-throughput processing is necessary. Here, we present a novel approach of combining multiplex ddPCR with experimental evolution for tracking genotype frequencies in different experimental populations over time. We found this method to be both precise and accurate for detecting and quantifying targets of interest, especially as compared to traditional PCR methods. The use of multiplex ddPCR to follow frequencies can be used for a wide range of experimental evolution applications.