analysis of genomic rearrangements associated with two variable antigen genes of Trypanosoma brucei.
analysis of genomic rearrangements associated with two variable antigen genes of Trypanosoma brucei.
复制标题
与布氏锥虫两个可变抗原基因相关的基因组重排分析。
DOI：
10.1093/nar/10.3.803
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发表时间：
1982
影响因子：
14.9
通讯作者：
R. Williams
中科院分区：
文献类型：
--
作者：
J. Young;J. Donelson;P. Majiwa;S. Z. Shapiro;R. Williams
布氏锥虫的一些可变表面糖蛋白（VSG）基因在表达时会发生重复和转座。我们在这里报告的cDNA编码两个VSG从ILtar 1的剧目的克隆。表达这些和其他抗原的锥虫的基因组的分析表明，在锥虫的表达克隆中没有编码8个VSG的序列的额外拷贝，并且揭示了类似于先前描述的来自该抗原库的另一个VSG的基因的重排。数据表明，重复并不伴随这些VSG基因的表达。不能排除转座至特定表达位点，但必须涉及比在表达时复制的VSG基因所观察到的更大的DNA片段，或移动至与先前侧翼序列具有更大同源性的区域。据推测，通过偶联复制和转座控制表达不足以解释选择单个VSG基因进行表达。
Some variable surface glycoprotein (VSG) genes of Trypanosoma brucei undergo duplication and transposition when they are expressed. We report here the cloning of cDNAs coding for two VSGs from the ILtar 1 repertoire. Analysis of the genomes of trypanosomes expressing these and other antigens shows that there is no additional copy of the sequences coding for eight VSG in expressing clones of trypanosomes, and reveals rearrangements analogous to those previously described for the gene for another VSG from this antigen repertoire. The data indicate that duplication does not accompany the expression of these VSG genes. Transposition to a specific expression site cannot be excluded, but would have to involve either a much larger segment of DNA, or movement to a region of much greater homology with the previous flanking sequences, than is observed for VSG genes that are duplicated when expressed. It is reasoned that the control of expression by coupled duplication and transposition is not sufficient to account for the selection of a single VSG gene for expression.