Encapsulation, cold storage, and growth of Hibiscus moscheutos nodal segments
Encapsulation, cold storage, and growth of Hibiscus moscheutos nodal segments
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木槿节段的封装、冷藏和生长
DOI：
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发表时间：
2006
影响因子：
3
通讯作者：
J. Preece
中科院分区：
文献类型：
--
作者：
T. West;M. Ravindra;J. Preece
从增殖的腋芽培养物中切下Hibiscusmoscheutos（哈代木槿）的节段，并包封在由50 mM CaCl 2硬化的高密度海藻酸钠中。4 mm长的节段生长良好，比8 mm长的节段更容易包裹。尽管节段生长与海藻酸钠的浓度无关，但由于2.75%海藻酸钠溶液产生的粘度，确定2.75%海藻酸钠产生最高质量的包封节段珠粒（足够的海藻酸盐涂层和易于使用）。当包封的片段在5°C下储存时，它们在光照或黑暗中不生长。在5°C黑暗中储存长达24周后，在正常孵育条件下的新鲜增殖培养基上的第一个月期间，根数以及根和芽伸长随着储存时间的增加而线性抑制。在两个单独的实验中，所有包封的节段在5°C储存24周后存活。事实上，80%的封装的哈代木槿节段在冷藏储存1年半（78周）后存活，并且在增殖培养基上3个月后，与未在5°C下储存或在5°C下储存24周的封装的节段相比，节段产生几乎相同长度的腋生芽，每个芽具有相同数量的腋生节。从封装和冷藏的'主巴尔的摩'节段的增长是更有力的比从'南贝儿'节段。
Nodal segments of Hibiscusmoscheutos (hardy hibiscus) were excised from proliferating axillary shoot cultures and encapsulated in high density sodium alginate hardened by 50 mM CaCl2. Nodal segments 4 mm long grew as well as and were easier to encapsulate than 8 mm long nodal segments. Although nodal segments grew regardless of the concentration of sodium alginate, 2.75% was determined to produce the highest quality encapsulated nodal segments beads (sufficient alginate coating and ease of use) because of the viscosity produced by the 2.75% sodium alginate solution. When encapsulated segments were stored at 5°C they did not grow in light or darkness. During the first month on fresh proliferation medium under normal incubation conditions following 5°C storage in the dark for up to 24 weeks, root number and root and shoot elongation were inhibited linearly as storage time increased. All encapsulated nodal segments survived 24 weeks of 5°C storage in two separate experiments. In fact, 80% of encapsulated hardy hibiscus nodal segments survived refrigerated storage for 1½ years (78 weeks) and after 3 months on proliferation medium, the nodal segments produced nearly the same length axillary shoots with the same number of axillary nodes per shoot as compared to encapsulated segments either not stored at 5°C or stored for 24 weeks at 5°C. Growth from encapsulated and cold-stored ‘Lord Baltimore’ nodal segments was more vigorous than from ‘Southern Belle’ nodal segments.