The influence of harvesting technique on endothelial preservation in saphenous veins.
The influence of harvesting technique on endothelial preservation in saphenous veins.
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DOI：
10.1016/0022-4804(92)90077-d
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发表时间：
1992-03
期刊：
影响因子：
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通讯作者：
D. Dries;S. F. Mohammad;S. Woodward;R. M. Nelson
中科院分区：
文献类型：
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作者：
D. Dries;S. F. Mohammad;S. Woodward;R. M. Nelson
为了研究准备大隐静脉作为冠状动脉旁路移植术的最佳条件，从连续 29 名接受冠状动脉旁路移植术的患者中获取隐静脉段。大隐静脉段分为三组。在第一组中，使用“非接触”技术收获 10 个隐静脉段，无需任何其他准备辅助工具。在第二组中，在使用 37°C 的平衡 pH 电解质溶液在 70-120 mm Hg 下扩张的同时，切除了 10 个隐静脉段。在由 10 个隐静脉段组成的第 III 组中，将硝化甘油 (1 μg/ml) 添加到第 II 组中使用的膨胀溶液中。在一项盲研究中，使用光学和扫描电子显微镜对隐静脉样本进行了评估，以估计三种收获技术对内皮细胞的保存情况。仅接受无扩张溶液的非接触式解剖技术的大隐静脉（第一组）显示出显着更好的内皮保存（P<0.005）。在收获期间使用我们的手术技术单独或与硝化甘油一起向隐静脉原位施用膨胀溶液似乎不能保护内皮完整性，并且可能是有害的。
To study optimal conditions of preparation of saphenous veins as coronary artery bypass grafts, segments of saphenous veins were obtained from 29 consecutive patients undergoing coronary artery bypass grafting. The saphenous vein segments were divided into three groups. In Group I, 10 saphenous vein segments were harvested using a “no-touch” technique without any other preparation aids. In Group II, 10 saphenous vein segments were removed while distended at 70–120 mm Hg with a balanced pH electrolyte solution at 37°C. In Group III, consisting of 10 saphenous vein segments, nitroglycerine (1 μg/ml) was added to the distending solution used in Group II. Samples of saphenous vein were assessed in a blind study using light and scanning electron microscopy to estimate endothelial cell preservation by the three harvest techniques. Saphenous veins receiving only a no-touch dissection technique without distention solution (Group I) revealed significantly better endothelial preservation (P< 0.005). The administration of distention solution alone, or with nitroglycerine, to saphenous veins inin situusing our operative technique during harvest does not appear to protect endothelial-integrity and may be harmful.