Thermococcus aciditolerans sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep- sea hydrothermal vent chimney in the Southwest Indian Ridge
Thermococcus aciditolerans sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep- sea hydrothermal vent chimney in the Southwest Indian Ridge
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嗜酸热球菌 sp.
DOI：
10.1099/ijsem.0.004934
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发表时间：
2021
影响因子：
2.8
通讯作者：
Wu Long-Fei
中科院分区：
文献类型：
--
作者：
Li Xue-Gong;Tang Hong-Zhi;Zhang Wei-Jia;Qi Xiao-Qing;Qu Zhi-Guo;Xu Jun;Wu Long-Fei
从西南印度洋脊水深2770 m的深海热液喷口烟囱中分离到一株极端嗜热、严格厌氧的古菌，命名为SY 113 T。菌株SY 113 T的富集和分离在85 °C和0.1 MPa下进行。SY 113 T菌株的细胞是不规则的运动球菌，具有周生鞭毛，直径通常为0.8-2.4 μm。在50至90 °C的温度（最佳为85 °C）和0.1-60 MPa的流体静压（最佳为27 MPa）下观察到生长。SY 113 T的细胞在pH 4.0-9.0（最适pH 5.5）和0.5- 5.5%（w/v;最适浓度3.0%NaCl）的NaCl浓度下生长。 SY 113 T是一株厌氧化能有机异养菌，能在酵母膏、胰蛋白胨、麦芽糖和淀粉等复杂蛋白质基质上生长。元素硫刺激生长，但不是其生长所必需的。基因组DNA的G+C含量为55.0mol%。对SY 113 T菌株的16 SrRNA序列进行系统发育分析，结果表明该菌株属于嗜热球菌属（Thermococcus）。根据其生理特性、平均核苷酸同源性和电子探针DNA-DNA杂交结果，我们提出了一个新种，命名为嗜酸热球菌Thermococcus aciditoleranssp.模式菌株为SY 113 T（=MCCC 1 K 04190 T =JCM 39083 T）。
A hyperthermophilic, strictly anaerobic archaeon, designated strain SY113T, was isolated from a deep-sea hydrothermal vent chimney on the Southwest Indian Ridge at a water depth of 2770 m. Enrichment and isolation of strain SY113Twere performed at 85 °C at 0.1 MPa. Cells of strain SY113Twere irregular motile cocci with peritrichous flagella and generally 0.8–2.4 µm in diameter. Growth was observed at temperatures between 50 and 90 °C (optimum at 85 °C) and under hydrostatic pressures of 0.1–60 MPa (optimum, 27 MPa). Cells of SY113Tgrew at pH 4.0–9.0 (optimum, pH 5.5) and a NaCl concentration of 0.5–5.5 % (w/v; optimum concentration, 3.0 % NaCl). Strain SY113Twas an anaerobic chemoorganoheterotroph and grew on complex proteinaceous substrates such as yeast extract and tryptone, as well as on maltose and starch. Elemental sulphur stimulated growth, but not obligatory for its growth. The G+C content of the genomic DNA was 55.0 mol%. Phylogenetic analysis of the 16S rRNA sequence of strain SY113Tshowed that the novel isolate belonged to the genusThermococcus. On the basis of physiological characteristics, average nucleotide identity values andin silicoDNA–DNA hybridization results, we propose a novel species, namedThermococcus aciditoleranssp. nov. The type strain is SY113T(=MCCC 1K04190T=JCM 39083T).