Increased new bone formation with a surface magnesium-incorporated deproteinized porcine bone substitute in rabbit calvarial defects
Increased new bone formation with a surface magnesium-incorporated deproteinized porcine bone substitute in rabbit calvarial defects
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DOI：
10.1002/jbm.a.34017
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发表时间：
2012-04-01
影响因子：
4.9
通讯作者：
Suh, Jo-Young
中科院分区：
文献类型：
--
作者：
Park, Jin-Woo;Ko, Hyuk-Jin;Suh, Jo-Young
本研究探讨了镁离子（Mg）掺入脱蛋白猪松质骨表面对兔颅骨缺损骨愈合的影响，以期利用镁的整合配体结合增强作用，并将其骨愈合能力与未处理的猪松质骨和脱蛋白牛骨（Bio-Oss）进行比较。采用碱性含镁溶液对含镁猪骨进行水热处理。采用扫描电镜、能量色散x射线能谱和x射线光电子能谱分析了样品的表面形貌和化学成分。在14只成年雄性新西兰大白兔的颅骨上制造了直径为7mm的缺陷，并用(1)未经处理的猪骨（PB）， (2) Bio-Oss和(3)含MG的猪骨（MG）填充。在植入后2周和4周用组织形态学方法评估新生骨百分比（NB%）。水热处理后，猪骨表面覆盖了一层大小约为100纳米的纳米结构。与其他各组相比，MG组支持更好的新骨形成。在早期愈合时间点，MG组在中心缺损区观察到骨导电性新骨形成。组织形态计量学分析显示，与未治疗的PB和Bio-Oss组相比，MG组在第4周的NB%显著增加（p < 0.05）。与未经处理的猪和牛异种骨移植相比，含镁猪骨表面纳米结构在兔颅骨骨缺损中能够快速形成新骨。(C) 2012 Wiley期刊公司[J]中国生物医学工程学报，2012。
This study investigated the effects of magnesium ion (Mg) incorporation into the surface of deproteinized porcine cancellous bone in the bone healing of rabbit calvarial defects with the expectation of utilizing the integrinligand binding enhancement effect of Mg, and compared its bone healing capacity with that of untreated porcine cancellous bone and deproteinized bovine bone (Bio-Oss). Hydrothermal treatment was performed to produce Mg-incorporated porcine bone using an alkaline Mg-containing solution. The surface morphology and chemical composition of the samples were investigated using scanning electron microscopy, energy-dispersive X-ray spectrometry, and X-ray photoelectron spectroscopy. Defects 7 mm in diameter were created in the calvaria of 14 adult male New Zealand White rabbits and were filled with (1) untreated porcine bone (PB), (2) Bio-Oss, and (3) Mg-containing porcine bone (MG). The percentage of newly formed bone (NB%) was evaluated histomorphometrically at 2 and 4 weeks after implantation. Hydrothermal treatment resulted in a Mg-containing surface in porcine bone covered with nanostructures similar to 100 nm in size. The MG group supported better new bone formation compared with the other groups. Osteoconductive new bone formation was observed in the central defect area in the MG group at an early healing time-point. Histomorphometric analysis revealed significantly greater NB% in the MG group when compared with the untreated PB and Bio-Oss groups at 4 weeks (p < 0.05). The Mg-incorporated porcine bone with surface nanostructures achieved rapid new bone formation in the osseous defects of rabbit calvaria compared with untreated xenografts of porcine and bovine origin. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.