Functional Blood Progenitor Markers in Developing Human Liver Progenitors.
Functional Blood Progenitor Markers in Developing Human Liver Progenitors.
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DOI：
10.1016/j.stemcr.2016.07.008
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发表时间：
2016-08-09
影响因子：
5.9
通讯作者：
Gouon-Evans V
中科院分区：
文献类型：
--
作者：
Goldman O;Cohen I;Gouon-Evans V
In the early fetal liver, hematopoietic progenitors expand and mature together with hepatoblasts, the liver progenitors of hepatocytes and cholangiocytes. Previous analyses of human fetal livers indicated that both progenitors support each other's lineage maturation and curiously share some cell surface markers including CD34 and CD133. Using the human embryonic stem cell (hESC) system, we demonstrate that virtually all hESC-derived hepatoblast-like cells (Hep cells) transition through a progenitor stage expressing CD34 and CD133 as well as GATA2, an additional hematopoietic marker that has not previously been associated with human hepatoblast development. Dynamic expression patterns for CD34, CD133, and GATA2 in hepatoblasts were validated in human fetal livers collected from the first and second trimesters of gestation. Knockdown experiments demonstrate that each gene also functions to regulate hepatic fate mostly in a cell-autonomous fashion, revealing unprecedented roles of fetal hematopoietic progenitor markers in human liver progenitors. Co-expression of hematopoietic markers CD34, CD133, and GATA2 in hESC-Hep cells Function of CD34, CD133, and GATA2 in hepatic specification of hESC-Hep cells Co-expression of CD34, CD133, and GATA2 in hepatoblasts from human fetal livers In this article, Gouon-Evans and colleagues show that virtually all hESC-derived hepatoblast-like cells (Hep cells) as human fetal hepatoblasts transition through a progenitor stage expressing the hematopoietic markers CD34, CD133, and GATA2. Knockdown experiments demonstrate that each gene regulates hepatic fate mostly in a cell-autonomous fashion, revealing unprecedented roles for hematopoietic progenitor markers in human liver progenitors.
