The effect of monovalent cations on the catalytic activity of thrombin.
The effect of monovalent cations on the catalytic activity of thrombin.
复制标题
单价阳离子对凝血酶催化活性的影响。
DOI：
10.1016/0003-9861(78)90199-6
复制
发表时间：
1978
影响因子：
3.9
通讯作者：
R. Lundblad
中科院分区：
文献类型：
--
作者：
E. Workman;R. Lundblad
利用多种底物研究了一价阳离子对凝血酶催化作用的影响。氯化钠非竞争性抑制凝血酶对α-对甲苯磺酰-L-精氨酸甲酯和α-N-苯甲酰基-L-精氨酸-对硝基苯胺的作用。以α-N-苯甲酰基-L-精氨酸乙酯为底物时，无抑制作用。无论是氯化钾还是氯化锂，抑制的程度都要小得多。在钠离子存在下，1-氯-3-甲苯磺酰胺基-7-氨基-L-2-庚酮对凝血酶的失活速率降低。这些结果被解释为显示了钠离子对活性部位组氨酸残基参与血栓催化的能力的特定影响。
The effect of monovalent cations on the catalytic action of thrombin has been examined utilizing a variety of substrates. Sodium chloride noncompetitively inhibited the action of thrombin on α-tosyl-l-arginine methyl ester and α-N-benzoyl-l-arginine-p-nitroanilide. No inhibition was noted when α-N-benzoyl-l-arginine ethyl ester was the substrate. The extent of inhibition was considerably less with either potassium chloride or lithium chloride. The rate of inactivation of thrombin by 1-chloro-3-tosylamido-7-amino-l-2-heptanone was reduced in the presence of sodium ions. The results are interpreted to show a specific effect of sodium ions on the ability of the active-site histidine residue to participate in thrombic catalysis.