Induction of Pluripotent Stem Cells from Primordial Germ Cells by Single Reprogramming Factors
Induction of Pluripotent Stem Cells from Primordial Germ Cells by Single Reprogramming Factors
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DOI：
10.1002/stem.1303
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发表时间：
2013-03-01
期刊：
影响因子：
5.2
通讯作者：
Suda, Toshio
中科院分区：
文献类型：
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作者：
Nagamatsu, Go;Kosaka, Takeo;Suda, Toshio
生殖细胞在基因表达模式和在培养中转化为多能干细胞的能力方面与多能干细胞相似。参与生殖细胞发育的因子也能够对体细胞进行重编程。这表明生殖细胞是研究体细胞重编程机制的有用工具。本研究分析了重编程因子在原始生殖细胞（PGCs）中的表达。PGCs表达Oct3/4、Sox2和c-Myc，但不表达Klf4。然而，在PGCs中表达的Klf2、Klf5、Essrb或Essrg可以在体细胞重编程过程中补偿Klf4。此外，PGCs可以通过感染任何已知的重编程因子（Oct3/4、Sox2、Klf4和c-Myc）转化为多能状态。这些细胞被命名为多能PGCs （mPGCs）。与体细胞重编程中体细胞起源的差异相反，我们假设重编程因子的基因表达水平在mpgc中会有所不同。通过比较外源表达c-Myc或L-Myc产生的mpgc的基因表达谱，确定了参与致瘤性和/或重编程效率调控的候选基因。干细胞2013；31:479 - 487
Germ cells are similar to pluripotent stem cells in terms of gene expression patterns and the capacity to convert to pluripotent stem cells in culture. The factors involved in germ cell development are also able to reprogram somatic cells. This suggests that germ cells are useful tools for investigating the mechanisms responsible for somatic cell reprograming. In this study, the expression of reprograming factors in primordial germ cells (PGCs) was analyzed. PGCs expressed Oct3/4, Sox2, and c-Myc but not Klf4. However, Klf2, Klf5, Essrb, or Essrg, which were expressed in PGCs, could compensate for Klf4 during somatic cell reprograming. Furthermore, PGCs could be converted to a pluripotent state by infection with any of the known reprogramming factors (Oct3/4, Sox2, Klf4, and c-Myc). These cells were designated as multipotent PGCs (mPGCs). Contrary to differences in the origins of somatic cells in somatic cell reprogramming, we hypothesized that the gene expression levels of the reprogramming factors would vary in mPGCs. Candidate genes involved in the regulation of tumorigenicity and/or reprogramming efficiency were identified by comparing the gene expression profiles of mPGCs generated by the exogenous expression of c-Myc or L-Myc. STEM CELLS 2013;31:479-487