Mitigation of nitrous oxide emissions from acidic soils by Bacillus amyloliquefaciens, a plant growth-promoting bacterium
Mitigation of nitrous oxide emissions from acidic soils by Bacillus amyloliquefaciens, a plant growth-promoting bacterium
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植物生长促进细菌解淀粉芽孢杆菌减少酸性土壤中一氧化二氮的排放
DOI：
10.1111/gcb.14025
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发表时间：
2018-06-01
影响因子：
11.6
通讯作者：
Zhuang, Xuliang
中科院分区：
文献类型：
--
作者：
Wu, Shanghua;Zhuang, Guoqiang;Zhuang, Xuliang
一氧化二氮（N2 O）是一种长期存在的温室气体，可导致大气化学的改变，并引起全球气候的变化。迄今为止，许多技术已被用于减少来自农田的N2 O排放，农田是N2 O的最重要来源之一。采用温室盆栽试验和微型血清瓶培养试验，研究了解淀粉芽孢杆菌（Bacillusamyloliquefaciens，BA）对蔬菜生长和N2 O排放的影响。土壤中添加BA促进了植物生长，提高了土壤pH值，增加了植物体内的全氮含量。同时降低了土壤中铵态氮（NH 4+）、硝态氮（NO3-）和全氮的含量。总的来说，BA的加入导致了50%的N2 O排放量的净减少与对照相比。定量PCR和DNA测序网络分析结果表明，BA通过显著减少氨氧化细菌数量而部分抑制硝化过程。同时，BA还促进了反硝化过程，主要是通过增加N2 O还原菌的数量来实现的。我们的微观实验结果提供了证据，有力地支持了在更严格控制的实验室条件下的上述发现。综上所述，我们的研究结果表明，BA具有促进植物生长和显著减少温室气体排放的双重作用，从而表明从环境中筛选出能够促进植物生长和减少温室微量气体的有益微生物的可能性。
Nitrous oxide (N2O) is a long-lived greenhouse gas that can result in the alteration of atmospheric chemistry and cause accompanying changes in global climate. To date, many techniques have been used to mitigate the emissions of N2O from agricultural fields, which represent one of the most important sources of N2O. In this study, we designed a greenhouse pot experiment and a microcosmic serum bottle incubation experiment using acidic soil from a vegetable farm to study the effects of Bacillus amyloliquefaciens (BA) on plant growth and N2O emission rates. The addition of BA to the soil promoted plant growth enhanced the soil pH and increased the total nitrogen (TN) contents in the plants. At the same time, it decreased the concentrations of ammonium (NH4+), nitrate (NO3-) and TN in the soil. Overall, the addition of BA resulted in a 50% net reduction of N2O emissions compared with the control. Based on quantitative PCR and the network analysis of DNA sequencing, it was demonstrated that BA partially inhibited the nitrification process through the significant reduction of ammonia oxidizing bacteria. Meanwhile, it enhanced the denitrification process, mainly by increasing the abundance of N2O-reducing bacteria in the treatment with BA. The results of our microcosm experiment provided evidence that strongly supported the above findings under more strictly controlled laboratory conditions. Taken together, the results of our study evidently demonstrated that BA has dual effects on the promotion of plant growth and the dramatic reduction of greenhouse emissions, thus suggesting the possibility of screening beneficial microbial organisms from the environment that can promote plant growth and mitigate greenhouse trace gases.