Organic-inorganic relationships, and immunohistochemical localization of amelogenins and enamelins in developing enamel.
Organic-inorganic relationships, and immunohistochemical localization of amelogenins and enamelins in developing enamel.
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牙釉质发育中釉原蛋白和牙釉质的有机-无机关系以及免疫组织化学定位。
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发表时间：
1986
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通讯作者：
E. Bonucci
中科院分区：
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作者：
Y. Hayashi;P. Bianco;H. Shimokawa;J. Termine;E. Bonucci
采用包埋后脱钙染色（PEDS）法和免疫组化蛋白a -金技术分别研究了小牛磨牙未成熟牙釉质的有机-无机关系，以及釉质原蛋白和釉质素在基质中的定位。在未成熟的釉质基质中，PEDS法显示出与未处理的晶体（晶体幽灵）在形状、位置和取向上相似的丝状和带状有机结构。免疫组化技术显示，淀粉原蛋白未被无机物掩盖，在未钙化和脱钙化切片中均有反应，金颗粒主要位于晶间区；相反，珐琅质被无机物质所掩盖，因为它们只在脱钙部分起反应，金颗粒有时与丝状和带状晶体幽灵密切相关。这些结果与先前的形态学研究结果一致，表明未成熟牙釉质的晶体是有机-无机结构，并与生化结果一致，表明淀粉原蛋白位于晶间区，搪瓷素位于晶间区。提出了一种假说，即珐琅质是未成熟珐琅质结晶幽灵的一部分。
The organic-inorganic relationships in calf molar immature enamel, and the localization of amelogenins and enamelins in its matrix, have been studied by post-embedding decalcification and staining (PEDS) method and the immunohistochemical protein A-gold technique, respectively. Filament- and ribbon-like organic structures similar in shape, location and orientation to untreated crystals (crystal ghosts) were shown by PEDS method in immature enamel matrix. Immunohistochemical technique showed that amelogenins were not masked by inorganic substance, because they were reactive both in undecalcified and decalcified sections, gold particles being mainly located at intercrystallite regions; on the contrary, enamelins were masked by inorganic substance because they were reactive only in decalcified sections, gold particles being sometimes closely related to filament- and ribbon-like crystal ghosts. These results are in agreement with those of previous morphologic studies, showing that crystals of immature enamel are organic-inorganic structures, and with biochemical findings suggesting that amelogenins are placed in intercrystallite regions and enamelins in crystallite regions. The hypothesis is advanced that enamelins are part of the crystal ghosts of immature enamel.